Proteome changes in rainbow trout (Oncorhynchus mykiss) fertilized eggs as an effect of triploidization heat-shock treatment

Animal Reproduction Science
Samad Bahrami BabaheydariSeyed Ali Johari

Abstract

The aim of the present study was to explore proteome changes in rainbow trout (Oncorhynchus mykiss) fertilized eggs as an effect of triploidization heat-shock treatment. Eggs and milt were taken from eight females and six males. The gametes were pooled to minimize the individual differences. After insemination, the eggs were incubated at 10°C for 10min. Half of the fertilized eggs were then subjected to heat shock for 10min submerged in a 28°C water bath to induce triploidy. The remainder were incubated normally and used as diploid controls. Three batches of eggs were randomly selected from each group and were incubated at 10-11°C under the same environmental conditions in hatchery troughs until the fry stage. Triplicate samples of 30 eggs (10 eggs per trough) from each group were randomly selected 1.5h post-fertilization for proteome extraction. Egg proteins were analyzed using two-dimensional electrophoresis (2-DE) and MALDI-TOF/TOF mass spectrometry. Based on the results from the statistical analyses, 15 protein spots were found to decrease significantly in abundance in heat-shock treated group and were selected for identification. Out of 15 protein spots showing altered abundance, 14 spots were successfully identified. All ...Continue Reading

References

Jun 3, 2004·Electrophoresis·Giovanni CandianoPier Giorgio Righetti
Dec 7, 2007·Animal Reproduction Science·Saeed Keyvanshokooh, Behrouz Vaziri
Mar 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Tamar ZivArie Admon
Apr 14, 2012·Journal of Proteomics·Pedro M RodriguesFlemming Jessen
Oct 23, 2014·Fish Physiology and Biochemistry·Gholamreza GhaediGholamreza Nikbakht Broujeni

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Citations

Aug 27, 2016·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Samad Bahrami BabaheydariSeyed Ali Johari
Nov 17, 2016·Fish Physiology and Biochemistry·Samad Bahrami BabaheydariSeyed Ali Johari

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