Proteome changes in renal cortex and medulla induced by dietary supplementation with inulin-type fructans in growing pigs

Journal of Animal Physiology and Animal Nutrition
Paulina RobakJacek Skomiał

Abstract

The aim of the study was to evaluate the influence of dietary supplementation with inulin extract from chicory root and dried chicory root on the protein profile of the renal cortex and medulla of growing pigs. The experiment was carried out on renal cortex and medulla tissue collected from 24 50-day-old PIC x Penarlan P76 crossbred piglets (males). Animals were divided into three dietary groups (n = 8) and fed with a control diet, diet supplemented with 2% inulin extract from chicory root and a diet supplemented with 4% dried chicory root. Kidney samples were collected after 40 days of feeding, and renal cortex and medulla proteins were separated by two-dimensional electrophoresis. Protein identification was performed using MALDI-TOF mass spectrometry. The diet supplemented with 2% chicory inulin induced significant expression changes of 20 and 26 protein spots in the renal cortex and medulla respectively. Supplementation with 4% dried chicory root triggered changes in the expression of 44 and 24 proteins in the renal cortex and medulla respectively. Both forms of chicory inulin-type fructans effectively affected the expression of proteins involved in energy metabolism, heat shock proteins and other chaperones, cytoskeletal an...Continue Reading

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Citations

Mar 22, 2020·Advances in Nutrition·Marie-Luise Puhlmann, Willem M de Vos

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