Proteome changes in the small intestinal mucosa of broilers (Gallus gallus) induced by high concentrations of atmospheric ammonia

Proteome Science
Jize ZhangHongfu Zhang

Abstract

Ammonia is a well-known toxicant both existing in atmospheric and aquatic system. So far, most studies of ammonia toxicity focused on mammals or aquatic animals. With the development of poultry industry, ammonia as a main source of contaminant in the air is causing more and more problems on broiler production, especially lower growth rate. The molecular mechanisms that underlie the negative effects of ammonia on the growth and intestine of broilers are yet unclear. We investigated the growth, gut morphology, and mucosal proteome of Arbor Acres broilers (Gallus gallus) exposed to high concentrations of atmospheric ammonia by performing a proteomics approach integrated with traditional methods. Exposure to ammonia interfered with the development of immune organ and gut villi. Meanwhile, it greatly reduced daily weight gain and feed intake, and enhanced feed conversion ratio. A total of 43 intestinal mucosal proteins were found to be differentially abundant. Up-regulated proteins are related to oxidative phosphorylation and apoptosis. Down-regulated proteins are related to cell structure and growth, transcriptional and translational regulation, immune response, oxidative stress and nutrient metabolism. These results indicated that...Continue Reading

References

Jun 1, 1992·Gastroenterology·M TsujiiN Sato
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C N OliverR A Floyd
Dec 1, 1994·Current Opinion in Cell Biology·J I Gordon, M L Hermiston
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·T P StosselS S Shapiro
May 5, 2001·Biochimica Et Biophysica Acta·A van der Flier, A Sonnenberg
Mar 15, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·John D CreminSharon E Fleming
Mar 28, 2003·The EMBO Journal·Yehudit BirgerMichael Bustin
Jan 30, 2004·Journal of Virology·Susan E CollinsKaren L Mossman
Apr 22, 2004·Biochimica Et Biophysica Acta·Bernhard KadenbachMaik Hüttemann
May 5, 2006·Fundamental & Clinical Pharmacology·M Mohamed Essa, P Subramanian
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Oct 13, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Renu N Jain, Linda C Samuelson
Sep 21, 2007·Biochimica Et Biophysica Acta·Gotaro IiizumiHiroaki Kawabata
Oct 11, 2007·BMC Genomics·Milka SokolovićWouter H Lamers
Jun 3, 2008·Biochimica Et Biophysica Acta·Qingchun Zhang, Yinsheng Wang
Jun 19, 2008·The Journal of Cell Biology·Yong TianSuzanne Jackowski
Jan 9, 2009·Amino Acids·J Marc Rhoads, Guoyao Wu
Jan 23, 2009·Nature Cell Biology·Michael WanzelMartin Eilers
Feb 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sarah NichollsBenjamin E Willcox
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laëtitia Le PottierJacques-Olivier Pers
Aug 14, 2009·Electrophoresis·Angel P DizDavid O F Skibinski
Jan 9, 2010·Journal of Biomedical Science·Jurandir F ComarAdelar Bracht
Aug 7, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mireille AndriamihajaFrançois Blachier
Aug 18, 2010·Genes & Development·Maxim A SkabkinTatyana V Pestova
Sep 28, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·A Di GarboP K Maini
Apr 14, 2011·Human Molecular Genetics·Yiguo ShenLouis J Ptácek
Jul 4, 2012·Molecular and Cellular Biology·Masahiro MoritaNahum Sonenberg
Nov 8, 2012·British Poultry Science·L SherlockD C Wathes

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Citations

Sep 5, 2019·Asian-Australasian Journal of Animal Sciences·Samiru Sudharaka WickramasuriyaJung Min Heo
Mar 20, 2019·Frontiers in Veterinary Science·Birendra Mishra, Rajesh Jha
Apr 4, 2019·PeerJ·Ádám SimonLevente Czeglédi
Jun 3, 2017·Proteome Science·Emily L O'ReillyP David Eckersall
May 24, 2017·Frontiers in Physiology·Hongjia OuyangQinghua Nie
Jun 23, 2020·BioMed Research International·Qiankun WangXiaoping Li
Mar 8, 2020·Environmental Science and Pollution Research International·Mohamed Ahmed FathiChunmei Li
Jun 30, 2019·Journal of Microbiological Methods·Ding-Hong JiaWei-Hong Peng
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Hongyu HanMinhong Zhang
Jul 3, 2021·Proteomes·Eleni I KatsarouAngeliki I Katsafadou

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Methods Mentioned

BETA
ELISAs
Protein Assay
TRET
PCR
electrophoresis

Software Mentioned

Blast2GO
Proteome Discover
SAS
MASCOT

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis