Proteome exploration to provide a resource for the investigation of Ganoderma lucidum

PloS One
Guo-Jun YuHui Sun

Abstract

Ganoderma lucidum is a basidiomycete white rot fungus that has been used for medicinal purposes worldwide. Although information concerning its genome and transcriptome has recently been reported, relatively little information is available for G. lucidum at the proteomic level. In this study, protein fractions from G. lucidum at three developmental stages (16-day mycelia, and fruiting bodies at 60 and 90 days) were prepared and subjected to LC-MS/MS analysis. A search against the G. lucidum genome database identified 803 proteins. Among these proteins, 61 lignocellulose degrading proteins were detected, most of which (49 proteins) were found in the 90-day fruiting bodies. Fourteen TCA-cycle related proteins, 17 peptidases, two argonaute-like proteins, and two immunomodulatory proteins were also detected. A majority (470) of the 803 proteins had GO annotations and were classified into 36 GO terms, with "binding", "catalytic activity", and "hydrolase activity" having high percentages. Additionally, 357 out of the 803 proteins were assigned to at least one COG functional category and grouped into 22 COG classifications. Based on the results from the proteomic and sequence alignment analyses, a potentially new immunomodulatory prote...Continue Reading

References

Dec 3, 1999·Applied and Environmental Microbiology·T M D'SouzaC A Reddy
Feb 15, 2000·Molecular Microbiology·S Shahinian, H Bussey
Apr 21, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D L LiuL Ma
Jan 22, 2002·Medical Mycology·C M Douglas
May 22, 2003·The Biochemical Journal·Chenguang ZhaoYipeng Qi
Aug 6, 2003·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Ming-Shi Shiao
Aug 13, 2003·Bioscience, Biotechnology, and Biochemistry·Thida SripuanHidehiko Kumagai
Aug 26, 2003·Advances in Biochemical Engineering/biotechnology·Albert SickmannHelmut E Meyer
Sep 13, 2003·BMC Bioinformatics·Roman L TatusovDarren A Natale
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Hanno Steen, Matthias Mann
Dec 14, 2004·Journal of Microbiological Methods·Alexander ScherlJacques Schrenzel
Aug 15, 2006·Phytochemistry·R Russell M Paterson
Mar 27, 2007·Nature Biotechnology·Monisha G ScottRobert E W Hancock
Aug 22, 2007·The American Journal of Chinese Medicine·Xuanwei ZhouKexuan Tang
Sep 19, 2007·Biotechnology Annual Review·Bojana BohLin Zhi-Bin
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Oct 11, 2008·Nature Biotechnology·Jay Shendure, Hanlee Ji
Oct 16, 2008·Natural Product Research·Chuan-Rui ZhangJian-Min Yue
May 12, 2009·New Biotechnology·Wilhelm J Ansorge
Jun 6, 2009·New Biotechnology·Yasumi HorimotoRickey Y Yada
Aug 5, 2009·International Immunopharmacology·Shailesh DudhgaonkarDaniel Sliva
Nov 27, 2009·Current Pharmaceutical Biotechnology·Bhagwan S SanodiyaP S Bisen
Oct 6, 2010·Applied Microbiology and Biotechnology·José Miguel P Ferreira de Oliveira, Leo H de Graaff
Oct 21, 2010·BMC Plant Biology·Javier TerolManuel Cercos
Mar 17, 2011·Advances in Clinical Chemistry·Kei OhnumaChikao Morimoto
Dec 2, 2011·Journal of Proteomics·Timothy A ReinhardtRandy E Sacco
Jan 24, 2012·Natural Product Research·Peng LiJian-Hua Xu

❮ Previous
Next ❯

Citations

Mar 28, 2016·The Protein Journal·Jameel R Al-ObaidiAbu Seman Idris
Feb 20, 2016·Electrophoresis·Jameel R Al-Obaidi
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shaza M ElhusseinyKhaled M Aboshanab

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
column chromatography
GlycosylTransferases
reverse transcription-PCR

Software Mentioned

Mascot
GraphPad Prism
Flex Analysis
ClustalX
Origin
Jalview

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.