Proteome profile of peripheral myelin in healthy mice and in a neuropathy model.

ELife
Sophie B SiemsHauke B Werner

Abstract

Proteome and transcriptome analyses aim at comprehending the molecular profiles of the brain, its cell-types and subcellular compartments including myelin. Despite the relevance of the peripheral nervous system for normal sensory and motor capabilities, analogous approaches to peripheral nerves and peripheral myelin have fallen behind evolving technical standards. Here we assess the peripheral myelin proteome by gel-free, label-free mass-spectrometry for deep quantitative coverage. Integration with RNA-Sequencing-based developmental mRNA-abundance profiles and neuropathy disease genes illustrates the utility of this resource. Notably, the periaxin-deficient mouse model of the neuropathy Charcot-Marie-Tooth 4F displays a highly pathological myelin proteome profile, exemplified by the discovery of reduced levels of the monocarboxylate transporter MCT1/SLC16A1 as a novel facet of the neuropathology. This work provides the most comprehensive proteome resource thus far to approach development, function and pathology of peripheral myelin, and a straightforward, accurate and sensitive workflow to address myelin diversity in health and disease.

References

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Citations

Sep 26, 2020·Frontiers in Cellular Neuroscience·Olaf JahnHauke B Werner
Aug 8, 2020·Cells·Arne Raasakka, Petri Kursula
Oct 9, 2020·Nature Communications·Nimrod Elazar, Elior Peles
Jan 12, 2021·Frontiers in Neuroscience·Matthew R SapioMichael J Iadarola
Nov 3, 2020·Frontiers in Cellular Neuroscience·Mithilesh Kumar Jha, Brett M Morrison
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Sophie B SiemsHauke B Werner
May 4, 2021·Neural Plasticity·Xiaodan WangDonghong Cui
May 29, 2021·NPJ Systems Biology and Applications·Tiago AzevedoEric R Gamazon
Mar 17, 2021·Annual Review of Neuroscience·Klaus-Armin Nave, Hauke B Werner
Sep 2, 2021·Developmental Cell·Ozge E Tasdemir-YilmazRosalind A Segal
Nov 4, 2021·Molecular Biology Reports·Sedigheh Momenzadeh, Mohammad-Saeid Jami

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Datasets Mentioned

BETA
GSE115930
PXD015960

Methods Mentioned

BETA
electrophoresis
density gradient centrifugation
RNA-Seq
electron microscopy
PCR
Protein Assay

Software Mentioned

MS
R package mfuzz
ISOQuant
UDMS E
UDMS
TOP3
R gplots
LAS AF lite
Tophat Aligner
Bioconductor R

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