Proteome profiling of embryo chick retina.

Proteome Science
Mina MizukamiYoshiaki Kiuchi

Abstract

Little is known regarding the molecular pathways that underlie the process of retinal development. The purpose of this study was to identify proteins which may be involved in development of retina. We used a proteomics-based approach to identify proteins that are up- or down-regulated during the development of the embryo chick retina. Two-dimensional gel electrophoresis was performed with the retina of embryo chicken, which was obtained from embryos of day 7 (ED7) and of day 11 (ED11). The protein spots showing significant differences were selected for identification by MALDI mass spectrometry. Thirteen proteins were differentially expressed; seven proteins were up-regulated in embryo retina of chicken at ED 11 and six proteins were down-regulated. Significant proteins were also evaluated in embryo day 15 (ED15). Some of identified proteins were known to regulate cell proliferation, cell death, transport, metabolism, organization and extracellular matrix, and others also included novel proteins. We identified thirteen proteins which differentially expressed in embryonal retina of chicken at day 7, as compared to the retina of embryo of day 11. They were various regulatory proteins for cellular signaling.

References

Jan 1, 1994·Experimental Eye Research·J ZwaanK W Lam
Aug 20, 1996·Brain Research. Developmental Brain Research·C K Mitchell, D A Redburn
Nov 18, 1996·The Journal of Comparative Neurology·H Hering, S Kröger
Oct 7, 1997·Molecular and Cellular Biology·D A BisgroveR Godbout
Nov 24, 1998·Acta Ophthalmologica Scandinavica·M HuB Ehinger
Mar 25, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D A Bisgrove, R Godbout
Aug 5, 2000·Progress in Retinal and Eye Research·R Adler
Nov 22, 2000·Molecular and Cellular Neurosciences·J WalkenhorstU Drescher
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·W LiuM Xiang
Feb 8, 2003·Genomics·JunQing QianGregory M Shackleford
Jun 28, 2003·Neurobiology of Disease·J H WeishauptM Bähr
Sep 10, 2003·Mechanisms of Development·Kiyo SakagamiKunio Yasuda
Feb 18, 2004·Protein and Peptide Letters·Petr G LokhovVladimir N Prozorovskii
Jun 7, 2005·Journal of Neurochemistry·Elena RommLynn D Hudson
Sep 21, 2005·Nature Neuroscience·Josana RodriguezPaola Bovolenta
Nov 2, 2005·Molecular and Cellular Biology·Dorota Skowronska-KrawczykJean-Marc Matter
Feb 2, 2006·Molecular and Cellular Biology·Albéna PramatarovaBrian W Howell
Jun 10, 2006·Development·Anne C von PhilipsbornMartin Bastmeyer
Jul 29, 2006·Developmental Biology·Ernesto F Moreira, Ruben Adler
Sep 29, 2006·Developmental Biology·Brian A TurnerBarbara L Hempstead
Feb 6, 2007·Molecular and Cellular Neurosciences·Samantha A OblanderSusann M Brady-Kalnay
Feb 28, 2007·Investigative Ophthalmology & Visual Science·Aneesh NeekhraM Cristina Kenney
Apr 13, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fumitaka OsakadaMasayo Takahashi

❮ Previous
Next ❯

Citations

Sep 27, 2012·Journal of Natural Products·Marc SlatteryJohn M Rimoldi
Jul 26, 2008·Proteome Science·Wolfgang E Reintsch, Craig A Mandato
Dec 17, 2008·Proteome Science·Sorcha FinneganWilliam J Curry
Feb 26, 2010·BMC Research Notes·Alison E BarnhillHeather West Greenlee
Jan 16, 2010·Proteomics·Paul BlakeleySimon J Hubbard
May 16, 2013·Molecular & Cellular Proteomics : MCP·Fabienne SouletAndreas Bikfalvi
Oct 31, 2009·Journal of Proteome Research·Els BonLennard J M Dekker

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

PDQuest
Quest
ProFound
PD

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.