Proteome profiling of triple negative breast cancer cells overexpressing NOD1 and NOD2 receptors unveils molecular signatures of malignant cell proliferation

BMC Genomics
Fernando J VellosoRicardo G Correa

Abstract

Triple negative breast cancer (TNBC) is a malignancy with very poor prognosis, due to its aggressive clinical characteristics and lack of response to receptor-targeted drug therapy. In TNBC, immune-related pathways are typically upregulated and may be associated with a better prognosis of the disease, encouraging the pursuit for immunotherapeutic options. A number of immune-related molecules have already been associated to the onset and progression of breast cancer, including NOD1 and NOD2, innate immune receptors of bacterial-derived components which activate pro-inflammatory and survival pathways. In the context of TNBC, overexpression of either NOD1or NOD2 is shown to reduce cell proliferation and increase clonogenic potential in vitro. To further investigate the pathways linking NOD1 and NOD2 signaling to tumorigenesis in TNBC, we undertook a global proteome profiling of TNBC-derived cells ectopically expressing each one of these NOD receptors. We have identified a total of 95 and 58 differentially regulated proteins in NOD1- and NOD2-overexpressing cells, respectively. We used bioinformatics analyses to identify enriched molecular signatures aiming to integrate the differentially regulated proteins into functional networks...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D A PeattieM Benasutti
Jun 27, 1998·The Journal of Biological Chemistry·J V McCarthyV M Dixit
May 18, 1999·The Journal of Biological Chemistry·N InoharaG Núñez
May 23, 2001·The Journal of Biological Chemistry·A MasudaY Yoshikai
Sep 5, 2002·Arthritis and Rheumatism·Markus A PenttinenKaisa Granfors
Dec 12, 2002·The Biochemical Journal·Philippe SoubeyranIvan Dikic
Mar 6, 2004·The Journal of Biological Chemistry·Bernard DegryseDavid J Loskutoff
May 11, 2004·EMBO Reports·Peter SteensgaardAndrea Musacchio
Sep 8, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·James D BrentonCarlos Caldas
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Nov 1, 2005·The Journal of Biological Chemistry·Dieter WeichartPhilip Rosenstiel
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jean da Silva CorreiaRichard J Ulevitch
Jul 18, 2006·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Tomoko MochizukiMitsutoshi Iwashita
Oct 13, 2006·Molecular and Cellular Biology·Maiko OgataKazunori Imaizumi
Nov 7, 2006·IUBMB Life·Zhimin Lu, Shuichan Xu
Dec 1, 2006·The Journal of Biological Chemistry·Wen-Xing DingXiao-Ming Yin
Dec 23, 2006·Cell Death and Differentiation·J da Silva CorreiaR J Ulevitch
Mar 14, 2007·The Biochemical Journal·Mark WindheimPhilip Cohen
Mar 16, 2007·Journal of Cell Science·Sylvie Legrand-PoelsJacques Piette
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jean da Silva CorreiaRichard Ulevitch
Apr 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Béatrice ChambraudEtienne Emile Baulieu
Aug 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rebecca DentSteven A Narod
Oct 30, 2007·The Journal of Biological Chemistry·Jae-Young KimJun Ninomiya-Tsuji
Oct 31, 2007·Immunity·Thirumala-Devi KannegantiGabriel Núñez
Dec 15, 2007·The EMBO Journal·Mizuho HasegawaNaohiro Inohara
Sep 27, 2008·The EMBO Journal·Minghao ZhangLaurence H Pearl
Dec 26, 2008·Molecular Biology of the Cell·Jessica L CrowleyElizabeth J Luna

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Citations

Mar 7, 2019·Bioscience Reports·Fernando J VellosoRicardo G Correa
Aug 25, 2020·Bioscience Reports·Qiaoyun WangZhixiang Zhuang
Oct 8, 2020·Biomedical Chromatography : BMC·Edouard C Nice
Jun 3, 2021·Biomedicines·Victoria Fernández-GarcíaLisardo Boscá
Jun 8, 2021·Molecular Therapy Oncolytics·Xiao-Li YangDa Fu

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Datasets Mentioned

BETA
PXD012542

Methods Mentioned

BETA
ubiquitination
proteomic profiling
protein folding
acetylation

Software Mentioned

GSEA ( Gene Set Enrichment Analysis )
GSEA
Metascape
Venny
MORPHEUS
MSstats
IPA®
Ingenuity® Pathway Analysis ( IPA® )
MS Office Excel
MaxQuant

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