Proteome response of dental pulp cells to exogenous FGF8

Journal of Proteomics
Rozaliya TsikandelovaNikolay Ishkitiev

Abstract

FGF8 specifies early tooth development by directing the migration of the early tooth founder cells to the site of tooth emergence. To date the effect of the FGF8 in adult dental pulp has not been studied. We have assessed the regenerative potential of FGF8 by evaluating changes in the proteome landscape of dental pulp following short- and long-term exposure to recombinant FGF8 protein. In addition, we carried out qRT PCR analysis to determine extracellular/adhesion gene marker expression and assessed cell proliferation and mineralization in response to FGF8 treatment. 2D and mass spectrometry data showed differential expression of proteins implicated in cytoskeleton/ECM remodeling and migration, cell proliferation and odontogenic differentiation as evidenced by the upregulation of gelsolin, moesin, LMNA, WDR1, PLOD2, COPS5 and downregulation of P4HB. qRT PCR showed downregulation of proteins involved in cell-matrix adhesion such as ADAMTS8, LAMB3 and ANOS1 and increased expression of the angiogenesis marker PECAM1. We have observed that, FGF8 treatment was able to boost dental pulp cell proliferation and to enhance dental pulp mineralization. Collectively, our data suggest that, FGF8 treatment could promote endogenous healing o...Continue Reading

Citations

Jan 11, 2019·International Journal of Oncology·Yilong HaoQianming Chen
Nov 8, 2020·Clinical Oral Investigations·Poliana Amanda Oliveira SilvaTaia Maria Berto Rezende
Dec 19, 2020·Journal of Proteomics·Mirna S FreireTaia M B Rezende
Nov 13, 2020·World Journal of Stem Cells·Jagadish HosmaniHarish C Chandramoorthy
Aug 7, 2021·Frontiers in Pharmacology·Keyue LiuBo Gao

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