Proteome-wide analysis of single-nucleotide variations in the N-glycosylation sequon of human genes.

PloS One
Raja MazumderRadoslav Goldman

Abstract

N-linked glycosylation is one of the most frequent post-translational modifications of proteins with a profound impact on their biological function. Besides other functions, N-linked glycosylation assists in protein folding, determines protein orientation at the cell surface, or protects proteins from proteases. The N-linked glycans attach to asparagines in the sequence context Asn-X-Ser/Thr, where X is any amino acid except proline. Any variation (e.g. non-synonymous single nucleotide polymorphism or mutation) that abolishes the N-glycosylation sequence motif will lead to the loss of a glycosylation site. On the other hand, variations causing a substitution that creates a new N-glycosylation sequence motif can result in the gain of glycosylation. Although the general importance of glycosylation is well known and acknowledged, the effect of variation on the actual glycoproteome of an organism is still mostly unknown. In this study, we focus on a comprehensive analysis of non-synonymous single nucleotide variations (nsSNV) that lead to either loss or gain of the N-glycosylation motif. We find that 1091 proteins have modified N-glycosylation sequons due to nsSNVs in the genome. Based on analysis of proteins that have a solved 3D ...Continue Reading

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D D Pless, W J Lennarz
Dec 1, 1992·Current Opinion in Cell Biology·G W Hart
Oct 29, 1991·Biochemistry·E W DavieW Kisiel
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K A KretzJ S O'Brien
Oct 1, 1993·Current Opinion in Biotechnology·C E Warren
Apr 1, 1994·Protein Science : a Publication of the Protein Society·R Tyler-CrossR B Harris
Jan 1, 1993·Journal of Inherited Metabolic Disease·N OkamotoY Kato
Jul 1, 1995·Protein Engineering·A Imberty, S Pérez
Jan 11, 2000·Nucleic Acids Research·S T SherryK Sirotkin
Mar 28, 2001·Science·P M RuddR A Dwek
Jun 26, 2001·Glycoconjugate Journal·A Kobata
Sep 1, 2001·Biochimie·H H Freeze, V Westphal
Sep 7, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T OhnishiK Tanamoto
Nov 14, 2001·The Journal of Biological Chemistry·Jean da Silva Correia, Richard J Ulevitch
Mar 28, 1996·Chemical Reviews·Raymond A. Dwek
May 10, 2003·Clinical and Diagnostic Laboratory Immunology·Takahiro OhnishiKen-Ichi Tanamoto
Jul 2, 2003·Zoological Science·Mizuki HinoHiroshi Hosoya
Feb 5, 2004·Proteomics·Jakob BunkenborgJacek R Wiśniewski
May 4, 2004·BMC Bioinformatics·Shandar AhmadAkinori Sarai
May 17, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Yong CuiWei Zheng
Jun 1, 2005·Nature Genetics·Guillaume VogtJean-Laurent Casanova
Jul 15, 2005·The New England Journal of Medicine·Daniela S Krause, Richard A Van Etten
Feb 25, 2006·Human Molecular Genetics·Patrice RollPierre Szepetowski
Jun 1, 1996·Clinical Molecular Pathology·F Dall'olio
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Dec 2, 2006·Acta Crystallographica. Section D, Biological Crystallography·Boris BrumshteinJoel L Sussman
Mar 9, 2007·Nature·Christopher GreenmanMichael R Stratton
May 1, 2007·Current Opinion in Genetics & Development·Guillaume VogtJean-Laurent Casanova
May 23, 2007·Nucleic Acids Research·Paul HortonKenta Nakai
Feb 29, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Patrick Braun
Apr 1, 2008·Rheumatology·A Alavi, J S Axford
Apr 26, 2008·Breast Cancer Research and Treatment·Lucia CostantinoNicoletta Fortunati
May 17, 2008·Cancer Research·Joseph N ContessaTheodore S Lawrence
May 22, 2008·Cancer Science·Yan-Yang ZhaoNaoyuki Taniguchi
Nov 28, 2008·Journal of Proteome Research·Danni L MeanyDaniel W Chan
Jan 8, 2009·Disease Markers·Rosa PeracaulaRafael de Llorens
Jan 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yue ChenYingming Zhao
Jul 15, 2009·Methods in Molecular Biology·Huaiyu Mi, Paul Thomas
Oct 20, 2009·Nature Methods·Jonas NilssonGöran Larson
Nov 17, 2009·Nucleic Acids Research·Eric W SayersJian Ye

❮ Previous
Next ❯

Citations

Sep 19, 2014·Nucleic Acids Research·Yang PanRaja Mazumder
Feb 11, 2014·The Journal of Biological Chemistry·Hsueh-Liang FuRafael Fridman
Oct 16, 2015·International Journal of Molecular Medicine·Dong Seon KimYoonsoo Hahn
Sep 19, 2015·Pflügers Archiv : European journal of physiology·Nis Borbye PedersenStine Falsig Pedersen
Dec 19, 2014·Proteomics. Clinical Applications·Radoslav Goldman, Miloslav Sanda
Feb 8, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H GockP J Cowan
Apr 27, 2013·Genomics, Proteomics & Bioinformatics·Konstantinos KaragiannisRaja Mazumder
Apr 20, 2016·Protoplasma·Markus LanghansTobias Meckel
Oct 19, 2016·Analytical Chemistry·Miloslav Sanda, Radoslav Goldman
Jan 30, 2015·BMC Bioinformatics·Dong Seon Kim, Yoonsoo Hahn
May 16, 2020·Plant Molecular Biology·Tianshu ChenZhi Hong
Oct 25, 2020·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Ana García-GarcíaLaia Alsina
Nov 2, 2021·Mass Spectrometry Reviews·Tai-Tu LinTujin Shi

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation

Software Mentioned

Predotar
iProClust
Ingenuity Pathway Analysis ( IPA )
STRING
2ANT
PolyPhen
NetNGlyc
WoLF PSORT
NetGlyc
IPA

Related Concepts

Related Feeds

Cardiovascular Biology of GLP-1

Glucagon-like peptide 1 (GLP-1) plays a role in glucose metabolism, energy homeostasis, and inflammation suppression. GLP-1 receptor signaling has been shown to impact cardiovascular function. This feed focuses on the role of GLP-1 and GLP-1 receptor agonists on cardiovascular biology.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.