Proteome-wide lysine acetylation in cortical astrocytes and alterations that occur during infection with brain parasite Toxoplasma gondii

PloS One
Anne BouchutWilliam J Sullivan

Abstract

Lysine acetylation is a reversible post-translational modification (PTM) that has been detected on thousands of proteins in nearly all cellular compartments. The role of this widespread PTM has yet to be fully elucidated, but can impact protein localization, interactions, activity, and stability. Here we present the first proteome-wide survey of lysine acetylation in cortical astrocytes, a subtype of glia that is a component of the blood-brain barrier and a key regulator of neuronal function and plasticity. We identified 529 lysine acetylation sites across 304 proteins found in multiple cellular compartments that largely function in RNA processing/transcription, metabolism, chromatin biology, and translation. Two hundred and seventy-seven of the acetylated lysines we identified on 186 proteins have not been reported previously in any other cell type. We also mapped an acetylome of astrocytes infected with the brain parasite, Toxoplasma gondii. It has been shown that infection with T. gondii modulates host cell gene expression, including several lysine acetyltransferase (KAT) and deacetylase (KDAC) genes, suggesting that the host acetylome may also be altered during infection. In the T. gondii-infected astrocytes, we identified ...Continue Reading

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Citations

Apr 12, 2016·Frontiers in Plant Science·Gaoyuan Song, Justin W Walley
Oct 30, 2015·Science Translational Medicine·William J Sullivan, Stacey D Gilk
Oct 21, 2017·Molecular Microbiology·Rama R YakubuNatalie C Silmon de Monerri
Nov 3, 2017·Neuron·Fabian Hosp, Matthias Mann

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Methods Mentioned

BETA
GTPase
acetylation
glycosylation
histone acetylation

Software Mentioned

Secretome
SORCERER
SEQUEST
IceLogo
WebLogo
DAVID

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