Proteome-wide Lysine Glutarylation Profiling of the Mycobacterium tuberculosis H37Rv

Journal of Proteome Research
Longxiang XieJianping Xie

Abstract

Lysine glutarylation, a new protein posttranslational modification (PTM), was recently identified and characterized in both prokaryotic and eukaryotic cells. To explore the distribution of lysine glutarylation in Mycobacterium tuberculsosis, by using a comprehensive method combining the immune affinity peptide enrichment by the glutaryl-lysine antibody with LC-MS, we finally identified 41 glutarylation sites in 24 glutarylated proteins from M. tuberculosis. These glutarylated proteins are involved in various cellular functions such as translation and metabolism and exhibit diverse subcellular localizations. Three common glutarylated proteins including 50S ribosomal protein L7/L12, elongation factor Tu, and dihydrolipoamide succinyltransferase are shared between Escherichia coli and M. tuberculosis. Moreover, comparison with other PTMs characterized in M. tuberculosis, 15 glutarylated proteins, are found to be both acetylated and succinylated. Notably, several stress-response-associated proteins including HspX are glutarylated. Our data provide the first analysis of M. tuberculosis lysine glutarylated proteins. Further studies on the role of the glutarylated proteins will unveil the molecular mechanisms of glutarylation underlyi...Continue Reading

References

Dec 22, 2005·Infection and Immunity·Shuangping Shi, Sabine Ehrt
Jan 24, 2007·Biochimica Et Biophysica Acta·Emmanuelle SaccoAnnaïk Quémard
Jun 10, 2008·Archives of Biochemistry and Biophysics·Brenda A Frankel, John S Blanchard
Jan 9, 2013·Journal of Proteome Research·Kai ZhangZhongyi Cheng
Aug 2, 2013·Journal of Proteome Research·Hiroki OkanishiSeiki Kuramitsu
May 31, 2014·Journal of Proteome Research·Jianyi PanFukun Zhao
Sep 3, 2014·Molecular & Cellular Proteomics : MCP·Fengying LiuFeng Ge
Dec 3, 2014·The International Journal of Biochemistry & Cell Biology·Longxiang XieJianping Xie
Jan 20, 2015·Current Opinion in Microbiology·Tippapha PisithkulDaniel Amador-Noguez
Feb 27, 2015·Molecular & Cellular Proteomics : MCP·Matthew D Hirschey, Yingming Zhao

❮ Previous
Next ❯

Citations

Apr 27, 2019·Molecular Omics·Hussam J Al-BarakatiDukka B Kc
Jan 5, 2018·Molecular & Cellular Proteomics : MCP·Hanyang DongKai Zhang
Jun 14, 2019·Human Reproduction·Yi-Min ChengTao Luo
Nov 12, 2019·Frontiers in Microbiology·Kate L HarveySteven P Djordjevic
Jul 13, 2021·Frontiers in Cell and Developmental Biology·Longxiang XieKeli Qian
May 18, 2018·Journal of Proteome Research·Charlotte GaviardJulie Hardouin
Oct 23, 2020·Journal of Proteome Research·Lijun DouLei Xu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.