Proteomic 2D DIGE profiling of human vascular endothelial cells exposed to environmentally relevant concentration of endocrine disruptor PCB153 and physiological concentration of 17β-estradiol.

Cell Biology and Toxicology
Quentin Felty

Abstract

Considering the recent studies that question previously reported cardio-protective effects of estrogen, there is a growing concern that endocrine disruptors may also contribute to the pathology of cardiovascular disease. PCB153 is one of the most commonly found polychlorinated biphenyls, and based on epidemiological studies, has been implicated in cardiovascular disease. The endocrine disruptor PCB153 has been reported to bind the estrogen receptor alpha, induce vessel formation, and increase the formation of reactive oxygen species in endothelial cells. Since PCB153-induced phenotypic changes are similar to estradiol, we postulated that PCB153 activates redox signaling pathways common to 17β-estradiol. Whether the effect of PCB153 on the proteome is comparable to 17β-estradiol is not known. Therefore we investigated the proteome of human microvascular endothelial cells exposed to PCB153 (100 ng/ml) for 24 h. Using 2D DIGE coupled to MALDI-time of flight (TOF)/TOF MS, we found 96 protein spots significantly (greater than 1.5-fold) modulated by experimental treatments. Mass spectrometry identified 11 of 13 protein spots with high confidence protein score CI that was greater than 95%. Of the identified proteins, lamin A/C and far...Continue Reading

References

May 31, 1979·Annals of the New York Academy of Sciences·M WassermannH J Miller
Aug 1, 1991·Journal of Interferon Research·J Pavlovic, P Staeheli
Oct 1, 1973·Archives of Pathology·N S Irey, H J Norris
Nov 1, 1970·Journal of Agricultural and Food Chemistry·J Bitman, H C Cecil
Apr 7, 1995·The Journal of Biological Chemistry·L BazarM Avigan
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·D L RobertsJ J Kim
Sep 1, 1997·American Journal of Industrial Medicine·P Gustavsson, C Hogstedt
May 10, 2000·The Journal of Biological Chemistry·D A CalderwoodM H Ginsberg
Oct 31, 2000·Circulation Research·Z G JinB C Berk
May 23, 2002·International Journal of Hygiene and Environmental Health·Bernhard HennigLarry W Robertson
Jul 13, 2002·Annals of the New York Academy of Sciences·Maria C CidHynda K Kleinman
Feb 13, 2003·The Journal of Biological Chemistry·Michael P MalakhovDong-Er Zhang
Mar 11, 2003·Menopause : the Journal of the North American Menopause Society·Eugenio ArteagaGuillermo Marshall
Apr 28, 2004·The American Journal of Pathology·Se-Hwa KimZorina S Galis
Jun 24, 2004·Circulation·John H NewmanUNKNOWN National Heart, Lung and Blood Institute/Office of Rare Diseases
Dec 24, 2004·The Journal of Biological Chemistry·Carolyn M KlingeRobert S Keynton
Dec 3, 2005·Cancer Research·Dean TantinPhillip A Sharp
Feb 3, 2006·Genes & Development·Carmen IvorraVicente Andrés
Feb 7, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jennifer C DaveyJoshua W Hamilton
Oct 16, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Eun Jin LimBernhard Hennig
Feb 19, 2008·Atherosclerosis·Quentin Felty, Nicole Porther
Nov 19, 2008·The Journal of Cell Biology·José María GonzálezVicente Andrés
Jan 14, 2009·The Journal of Cell Biology·Ashraf N MalhasDavid J Vaux
Sep 26, 2009·Journal of Proteome Research·Jean-Paul LasserreTorsten Bohn

❮ Previous
Next ❯

Citations

Aug 16, 2011·Journal of Proteomics·Jayanth Ramadoss, Ronald R Magness
Oct 26, 2011·Toxicon : Official Journal of the International Society on Toxinology·Øyvind L BuskMorten Sørlie
Oct 25, 2014·Molecular Neurobiology·Brenda LunaDeodutta Roy
Aug 8, 2018·Medical Sciences : Open Access Journal·Andrea AvecillaVincent Avecilla
Apr 19, 2021·Toxicology and Applied Pharmacology·Yi SunWenchang Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.