Proteomic Alterations in Aqueous Humor From Patients With Primary Open Angle Glaucoma.

Investigative Ophthalmology & Visual Science
Shruti SharmaAshok Sharma

Abstract

Primary open angle glaucoma (POAG) is the most prevalent form of glaucoma, accounting for approximately 90% of all cases. The aqueous humor (AH), a biological fluid in the anterior and posterior chambers of the eye, is involved in a multitude of functions including the maintenance of IOP and ocular homeostasis. This fluid is very close to the pathologic site and is also known to have a significant role in glaucoma pathogenesis. The purpose of this study was to identify proteomic alterations in AH from patients with POAG. AH samples were extracted from 47 patients undergoing cataract surgery (controls: n = 32; POAG: n = 15). Proteomic analysis of the digested samples was accomplished by liquid-chromatography-mass spectrometry. The identified proteins were evaluated using a variety of statistical and bioinformatics methods. A total of 33 proteins were significantly altered in POAG subjects compared with the controls. The most abundant proteins in POAG subjects are IGKC (13.56-fold), ITIH4 (4.1-fold), APOC3 (3.36-fold), IDH3A (3.11-fold), LOC105369216 (2.98-fold). SERPINF2 (2.94-fold), NPC2 (2.88-fold), SUCLG2 (2.70-fold), KIAA0100 (2.29-fold), CNOT4 (2.23-fold), AQP4 (2.11-fold), COL18A1 (2.08-fold), NWD1 (2.07-fold), and TMEM120...Continue Reading

References

Dec 15, 2000·Progress in Neurobiology·J L VeneroJ Cano
Dec 20, 2003·The EMBO Journal·Alexander G MarnerosBjorn R Olsen
Sep 4, 2004·American Journal of Human Genetics·Xiaodong JiaoJ Fielding Hejtmancik
Jun 3, 2005·The Journal of Biological Chemistry·Kate E GregoryLynn Y Sakai
Jun 25, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Merja HurskainenTaina Pihlajaniemi
Mar 15, 2006·Anais Da Academia Brasileira De Ciências·Maria Rita Passos-BuenoKatia R M Leite
Mar 6, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Rajpal S KashyapHatim F Daginawala
May 14, 2010·Investigative Ophthalmology & Visual Science·Uttio Roy ChowdhuryMichael P Fautsch
Jul 24, 2010·PloS One·Chun-hong XiaXiaohua Gong
Jul 30, 2010·Journal of Proteome Research·A IzzottiS C Saccà
Oct 12, 2010·Experimental Eye Research·Zachary T ReschMichael P Fautsch
Aug 1, 2007·Proteomics. Clinical Applications·Franz H GrusNorbert Pfeiffer
Jun 19, 2012·Journal of Proteome Research·Tae Wan KimKwang Pyo Kim
Jun 12, 2013·Proteomics·Richard D SembaJennifer E Van Eyk
Sep 5, 2013·Investigative Ophthalmology & Visual Science·Yutao LiuMichael A Hauser
Sep 24, 2013·Progress in Retinal and Eye Research·Sarah F JanssenArthur A B Bergen
Feb 1, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Thuy Linh TranSteffen Heegaard
Sep 23, 2014·Cold Spring Harbor Perspectives in Medicine·Ryan Wang, Janey L Wiggs
May 20, 2016·Proteomics·Anna A KliuchnikovaSergei A Moshkovskii
Oct 28, 2016·PloS One·Martha Andrea KaeslinAlbert Neutzner
Dec 29, 2016·Progress in Retinal and Eye Research·Teresia CarreonSanjoy K Bhattacharya
Feb 23, 2017·Progress in Retinal and Eye Research·Ian D DanfordJoan M O'Brien
Mar 9, 2017·Expert Review of Proteomics·Sebastian FunkeFranz H Grus
Apr 27, 2017·Developments in Ophthalmology·Dawn LimPaul Chew
Jun 5, 2017·Lancet·Jost B JonasSonghomitra Panda-Jonas

❮ Previous
Next ❯

Citations

Jun 27, 2020·Diagnostics·Andrés Fernández-Vega CuetoHéctor González-Iglesias
Nov 30, 2018·International Journal of Molecular Medicine·Hongwei WangYong Ji
Sep 29, 2019·International Journal of Molecular Sciences·Hannah YoungbloodShruti Sharma
Nov 22, 2020·Proteomes·Sai Karthik KodeboyinaAshok Sharma
Feb 19, 2021·Journal of Cellular and Molecular Medicine·Aihua LiuLijie Dong
May 30, 2021·Translational Research : the Journal of Laboratory and Clinical Medicine·Mary Ann Stepp, A Sue Menko
Jul 3, 2021·Molecular & Cellular Proteomics : MCP·Xiaoyan LiuWei Sun
Aug 28, 2021·Biology·Andrés Fernández-Vega CuetoHéctor González-Iglesias
Oct 5, 2021·Lipids in Health and Disease·Parth A PatelAshok Sharma

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation

Software Mentioned

Proteome Discoverer
IPA
R Project for Statistical Computing
Percolator
Ingenuity Pathway Analysis ( IPA )
SequestHT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.