Proteomic analyses of monocyte-derived macrophages infected with human immunodeficiency virus type 1 primary isolates from Hispanic women with and without cognitive impairment.

Journal of Neurovirology
Dianedis Toro-NievesL M Meléndez

Abstract

The signature for human immunodeficiency virus type 1 (HIV-1) neurovirulence remains a subject of intense debate. Macrophage viral tropism is one prerequisite but others, including virus-induced alterations in innate and adaptive immunity, remain under investigation. HIV-1-infected mononuclear phagocytes (MPs; perivascular macrophages and microglia) secrete toxins that affect neurons. The authors hypothesize that neurovirulent HIV-1 variants affect the MP proteome by inducing a signature of neurotoxic proteins and thus affect cognitive function. To test this hypothesis, HIV-1 isolates obtained from peripheral blood of women with normal cognition (NC) were compared to isolates obtained from women with cognitive impairment (CI) and to the laboratory adapted SF162, a spinal fluid R5 isolate from a patient with HIV-1-associated dementia. HIV-1 isolates were used to infect monocyte-derived macrophages (MDMs) and infection monitored by secreted HIV-1 p24 by enzyme-linked immunosorbent assay (ELISA). Cell lysates of uninfected and HIV-1-infected MDMs at 14 days post infection were fractionated by cationic exchange chromatography and analyzed by surface enhanced laser desorption ionization time of flight (SELDI-TOF) using generalized e...Continue Reading

References

Feb 26, 2000·Science·S Gartner
Mar 16, 2000·Seminars in Neurology·J C McArthurO Selnes
Mar 14, 2001·Neuroscience·M Y AksenovW R Markesbery
Apr 18, 2002·AIDS Research and Human Retroviruses·M A ArroyoL M Meléndez-Guerrero
Oct 24, 2002·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Eric AndersonHoward E Gendelman
Dec 18, 2002·The Journal of Experimental Medicine·Christian StehlikJohn C Reed
Sep 10, 2003·Journal of Leukocyte Biology·Sharon M WahlNancy Vazquez
Sep 10, 2003·Journal of Leukocyte Biology·ChuHee LeeRonald G Collman
Jan 27, 2004·Journal of Neuroimmunology·Kimberly A CarlsonHoward E Gendelman
Jun 23, 2004·Neurobiology of Disease·Praveen BallabhMaiken Nedergaard
Dec 8, 2004·Journal of Neuroimmunology·Justin C McArthur
Jan 5, 2005·Nature Reviews. Immunology·Francisco González-Scarano, Julio Martín-García
Mar 15, 2005·The Journal of Biological Chemistry·Qiang XieJoseph A Bonanno
May 11, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Georgette D KanmogneYuri Persidsky
Sep 29, 2006·Journal of Leukocyte Biology·Luis E MunozUdo S Gaipl
Aug 7, 2007·International Review of Neurobiology·Ashley ReynoldsHoward E Gendelman
Oct 24, 2007·Journal of Neuroimmunology·Juliana Pérez LaspiurLoyda M Meléndez

❮ Previous
Next ❯

Citations

May 25, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Amanda BrownValerie Wojna
Dec 15, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Loyda M MeléndezDianedis Toro-Nieves
Jun 14, 2012·PloS One·Eillen J Rodriguez-FrancoLoyda M Melendez
Jun 3, 2015·Proteomics. Clinical Applications·Maire Rose Donnelly, Pawel Ciborowski
Nov 26, 2010·Antiviral Research·Julia J M EekelsBen Berkhout
Dec 8, 2010·Proteomics. Clinical Applications·Stephanie Kraft-TerryLoyda M Meléndez
May 11, 2010·Genomics, Proteomics & Bioinformatics·Lijun ZhangHonghao Zhou
Apr 2, 2021·Biopolymers·Darya Marchany-RiveraJuan López-Garriga

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.