DOI: 10.1101/501080Dec 19, 2018Paper

Proteomic Analyses of Morphological Variants of Borrelia burgdorferi Shed New Light on Persistence Mechanisms: Implications for Pathogenesis, Diagnosis and Treatment

BioRxiv : the Preprint Server for Biology
Jie Feng, Ying Zhang

Abstract

Borrelia burgdorferi causes Lyme disease, which is the most common vector borne disease in the United States and Europe. Although 2 to 4 week antibiotic treatment for Lyme disease is effective in the majority of cases, about 10 to 20% patients suffer from prolonged post-treatment Lyme disease syndrome (PTLDS). While the mechanisms of PTLDS are unclear, persisting organisms not killed by current Lyme antibiotics has been suggested as a possible explanation. B. burgdorferi can spontaneously develop different morphological variant forms under stress or in stationary phase with increased persistence to antibiotics. To shed light on the possible mechanisms by which these variant forms develop persistence, here, we isolated three B. burgdorferi forms, log phase spirochetal form, stationary phase planktonic form, and stationary phase aggregated biofilm like microcolony form. We showed that the two separated stationary phase forms especially microcolony form have more persistence to antibiotics than the log phase spirochetal form. Then, we performed mass spectrometry (MS/MS) analysis to determine the proteomic profiles of the three different forms to reveal the mechanisms of persistence in B. burgdorferi. We identified 1023 proteins in...Continue Reading

Related Concepts

Antibiotics
Birth
Borrelia burgdorferi
Genetic Vectors
Metabolism
Plankton
Mass Spectrometry
Up-Regulation (Physiology)
Vaccines
Virulence

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