Proteomic Analysis and NIR-II Imaging of MCM2 Protein in Hepatocellular Carcinoma

Journal of Proteome Research
Jing YangXuechuan Hong

Abstract

Targeted therapy of hepatocellular carcinoma (HCC) is essential for improved therapies. Therefore, identification of key targets specifically to HCC is an urgent requirement. Herein, an iTRAQ quantitative proteomic approach was employed to identify differentially expressed proteins in HCC tumor tissues. Of the upregulated tumor-related proteins, minichromosome maintenance 2 (MCM2), a DNA replication licensing factor, was one of the most significantly altered proteins, and its overexpression was confirmed using tissue microarray. Clinicopathological analysis of multiple cohorts of HCC patients indicated that overexpression of MCM2 was validated in 89.8% tumor tissues and strongly correlated with clinical stage. Furthermore, siRNA-mediated repression of MCM2 expression resulted in significant suppression of the HepG2 cell cycle and proliferation through the cyclin D-dependent kinases (CDKs) 2/7 pathway. Finally, the first small molecule-based MCM2-targeted NIR-II probe CH1055-MCM2 was concisely generated and subsequently evaluated in mice bearing HepG2 xenografts. The excellent imaging properties such as good tumor uptake and high tumor contrast and specificity were achieved in the small animal models. This analytical strategy ca...Continue Reading

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Citations

Jul 28, 2018·Advanced Healthcare Materials·Xiaodong ZengXuechuan Hong
Feb 12, 2019·Chemical Communications : Chem Comm·Xiaodong ZengYuling Xiao
Dec 4, 2019·Advanced Healthcare Materials·Hui ZhouXuechuan Hong
Feb 20, 2020·Chemical Communications : Chem Comm·Qianqian LiYuling Xiao
Mar 20, 2020·Molecular Oncology·Xiao ZhangXiaoli Lan
Feb 19, 2019·Chemical Science·Jiacheng LinXuechuan Hong
Nov 7, 2019·Frontiers in Chemistry·Yizhou LiAixi Yu
Sep 29, 2020·Advanced Optical Materials·Chunrong QuXuechuan Hong
Nov 10, 2021·The Journal of Pharmacology and Experimental Therapeutics·Yaotian HuJian Wang

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