Proteomic analysis and purification of an unusual germin-like protein with proteolytic activity in the latex of Thevetia peruviana

Planta
Cleverson Diniz Teixeira de FreitasMárcio V Ramos

Abstract

The latex from Thevetia peruviana is rich in plant defense proteins, including a 120 kDa cysteine peptidase with structural characteristics similar to germin-like proteins. More than 20,000 plant species produce latex, including Apocynaceae, Sapotaceae, Papaveraceae and Euphorbiaceae. To better understand the physiological role played by latex fluids, a proteomic analysis of Thevetia peruviana (Pers.) Schum latex was performed using two-dimensional gel electrophoresis and mass spectrometry. A total of 33 proteins (86 %) were identified, including storage proteins, a peptidase inhibitor, cysteine peptidases, peroxidases and osmotins. An unusual cysteine peptidase, termed peruvianin-I, was purified from the latex by a single chromatographic step involving gel filtration. The enzyme (glycoprotein) was inhibited by E-64 and iodoacetamide and exhibited high specific activity towards azocasein (K m 17.6 µM), with an optimal pH and temperature of 5.0-6.0 and 25-37 °C, respectively. Gel filtration chromatography, two-dimensional gel electrophoresis, and mass spectrometry revealed that peruvianin-I possesses 120 kDa, pI 4.0, and six subunits (20 kDa). A unique N-terminal amino acid sequence was obtained to oligomer and monomers of peruv...Continue Reading

References

Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Aug 10, 2001·Indian Journal of Experimental Biology·D Patnaik, P Khurana
Sep 13, 2002·Progress in Biophysics and Molecular Biology·Daniel J MüllerKurt Anderson
Mar 16, 2005·Analytical Biochemistry·Tatsuya MasukoYuan C Lee
Mar 8, 2006·Molecular Nutrition & Food Research·Jesus F CrespoStephan Scheurer
Apr 11, 2006·Annual Review of Phytopathology·L C van LoonC M J Pieterse
Feb 1, 1977·Plant Physiology·C N Giannopolitis, S K Ries
Sep 25, 2007·Plant Physiology and Biochemistry : PPB·Cleverson Diniz T FreitasMárcio Viana Ramos
Dec 19, 2007·The New Phytologist·William F Pickard
May 23, 2008·Planta medica·André Domsalla, Matthias F Melzig
Nov 4, 2008·Trends in Plant Science·Jillian M HagelPeter J Facchini
Nov 4, 2008·Toxicon : Official Journal of the International Society on Toxinology·Tatiana M AlbuquerqueMárcio V Ramos
Feb 22, 2011·Plant Physiology and Biochemistry : PPB·Cleverson Diniz Teixeira de FreitasMárcio Viana Ramos
Jul 25, 2013·Current Opinion in Plant Biology·Claudia SchelerJeremy Astier
May 23, 2014·Biochemical and Biophysical Research Communications·Xi ChengSiyi Pan

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Citations

Oct 21, 2016·Plant Physiology and Biochemistry : PPB·Cleverson D T FreitasMárcio V Ramos
Feb 10, 2021·Journal of Bioscience and Bioengineering·Andrea Y MansillaEnrique Martínez Campos
Jul 30, 2017·International Journal of Biological Macromolecules·Cleverson D T FreitasMárcio V Ramos
Jan 10, 2019·International Journal of Biological Macromolecules·Wallace T da CruzCleverson D T Freitas

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