Proteomic analysis dissects the impact of nodulation and biological nitrogen fixation on Vicia faba root nodule physiology

Plant Molecular Biology
Beate ThalHolger Eubel

Abstract

Symbiotic nitrogen fixation in root nodules of legumes is a highly important biological process which is only poorly understood. Root nodule metabolism differs from that of roots. Differences in root and nodule metabolism are expressed by altered protein abundances and amenable to quantitative proteome analyses. Differences in the proteomes may either be tissue specific and related to the presence of temporary endosymbionts (the bacteroids) or related to nitrogen fixation activity. An experimental setup including WT bacterial strains and strains not able to conduct symbiotic nitrogen fixation as well as root controls enables identification of tissue and nitrogen fixation specific proteins. Root nodules are specialized plant organs housing and regulating the mutual symbiosis of legumes with nitrogen fixing rhizobia. As such, these organs fulfill unique functions in plant metabolism. Identifying the proteins required for the metabolic reactions of nitrogen fixation and those merely involved in sustaining the rhizobia:plant symbiosis, is a challenging task and requires an experimental setup which allows to differentiate between these two physiological processes. Here, quantitative proteome analyses of nitrogen fixing and non-nitro...Continue Reading

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Citations

Jan 12, 2019·Plant, Cell & Environment·Willian Batista-SilvaTatjana M Hildebrandt
Apr 27, 2019·Molecular & Cellular Proteomics : MCP·Nils RugenHolger Eubel
Jun 25, 2019·Briefings in Functional Genomics·Twinkle Chaudhary, Pratyoosh Shukla
Sep 15, 2019·The Plant Journal : for Cell and Molecular Biology·Philippe FuchsMarkus Schwarzländer
Jul 1, 2019·Molecular & Cellular Proteomics : MCP·Nils RugenHolger Eubel
Feb 23, 2020·Current Biology : CB·Kerstin SchäferChris Carrie
May 10, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Nils RugenHolger Eubel
Mar 27, 2021·The Plant Cell·Niklas KluschHans-Peter Braun

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Datasets Mentioned

BETA
PXD008548

Methods Mentioned

BETA
Protein Assay
acetylations
PCA

Software Mentioned

Proteome Discoverer
FASTA
Mascot
Perseus
Andromeda
MaxQuant
Max Quant

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