Proteomic analysis for tissues and liquid from bonghan ducts on rabbit intestinal surfaces

Journal of Acupuncture and Meridian Studies
Soo Jae LeeKwang-Sup Soh

Abstract

Research on the Bonghan system has recently prompted great interest in the theory proposed by Bong Han Kimin in the early 1960s. In order to study the biochemical characteristics of the Bonghan system, we analyzed Bonghan ducts (BHD) on the surface of rabbit intestines and characterized the liquid in the BHD at the level of the proteome. Proteomic analysis was performed using nano LC-ESI MS/MS. Using a solution digestion technique, we identified 70 different proteins in the liquid of the BHD. We used gel-based digestion to analyze the BHD itself and our results showed the presence of 207 proteins. We used these proteins to analyze gene ontology (GO) to yield insights into biological processes, molecular functions and cellular compartmentalization. Remarkably, GO clustering showed high concentrations of proteins involved in metabolism. These proteins are not usually found in blood, lymph or blood vessels, and thus can be useful for characterizing BHD. It is worth studying their association with stem cells, especially mesenchymal stem cells, cancer cells and myeloid cells.

References

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Citations

Dec 15, 2010·The Journal of Membrane Biology·Jae-Hong ChoiPan Dong Ryu
Jul 17, 2010·Journal of Acupuncture and Meridian Studies·Jung Sun YooKwang-Sup Soh
Jul 16, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Byung-Cheon LeeSeung Zhoo Yoon
Aug 13, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Sang Hyun ParkYeon-Hee Ryu
Jun 14, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Hyub HuhSeung Zhoo Yoon
Jan 9, 2016·Journal of Acupuncture and Meridian Studies·Hye-Rie LeeSang-Suk Lee
Jun 14, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Ding-Jun CaiFan-Rong Liang
Jul 7, 2011·Connective Tissue Research·Jong Hyun JungKwang-Sup Soh
Jul 8, 2015·Stem Cells and Development·Rajani RaiByoung S Kwon
Sep 22, 2015·Journal of Pharmacopuncture·Miroslav Stefanov, Jungdae Kim
Aug 29, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Kwang-Sup SohYeon Hee Ryu
Dec 3, 2013·Journal of Acupuncture and Meridian Studies·Hyun-Jung HanKwang-Sup Soh
Jul 19, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Jun-Ling LiuBing Zhu
Jul 17, 2010·Journal of Acupuncture and Meridian Studies·Byung-Cheon LeeKwang-Sup Soh
Jul 17, 2010·Journal of Acupuncture and Meridian Studies·Hyun-Jung HanKwang-Sup Soh
Jun 28, 2011·Journal of Acupuncture and Meridian Studies·So Rim KimPan Dong Ryu
Aug 26, 2014·Journal of Acupuncture and Meridian Studies·Kyoung-Hee Bae, Kwang-Sup Soh
Dec 3, 2013·Journal of Acupuncture and Meridian Studies·Miroslav StefanovMin-Ho Nam
Jul 17, 2010·Journal of Acupuncture and Meridian Studies·Byung-Cheon Lee, Kwang-Sup Soh
Jul 17, 2010·Journal of Acupuncture and Meridian Studies·Kwang-Sup Soh
Jul 17, 2010·Journal of Acupuncture and Meridian Studies·Vyacheslav OgayKwang-Sup Soh
Jun 6, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Jaekwan LimYeon Hee Ryu
Mar 1, 2009·Journal of Acupuncture and Meridian Studies·Byung-Cheon LeeKwang-Sup Soh
Oct 9, 2012·Journal of Acupuncture and Meridian Studies·Miroslav Stefanov, Jungdae Kim
Dec 25, 2016·Journal of Acupuncture and Meridian Studies·Roshanak GhodsMohammad Hossein Ayati
Nov 26, 2019·Journal of Acupuncture and Meridian Studies·Lígia Rebelo Gomes, Pedro Leão
Jan 9, 2016·The Journal of the American Osteopathic Association·Bruno ChiklyJörgen Quaghebeur

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