Abstract
Gnathostoma spinigerum is the causative agent of human gnathostomiasis. The advanced third stage larva (AL3) of this nematode can migrate into the subcutaneous tissues, including vital organs, often producing severe pathological effects. This study performed immuno-proteomic analysis of antigenic spots, derived from G. spinigerum advanced third stage larva (GSAL3) and recognized by human gnathostomiasis sera, using two-dimensional (2-DE) gel electrophoresis based-liquid chromatography/tandem mass spectrometry (LC/MS-MS), and followed by the aid of a database search. The crude GSAL3 extract was fractionated using IPG strips (pH 3-11NL) and followed by SDS-PAGE in the second dimension. Each gel was stained with colloidal Coomassie blue or was electro-transferred onto a nitrocellulose membrane and probed with gnathostomiasis human sera by immunoblotting. Individual Coomassie-stained protein spots corresponding to the antigenic spots recognized by immunoblotting were excised and processed using LC/MS-MS. Of the 93 antigenic spots excised, 87 were identified by LC/MS-MS. Twenty-seven protein types were found, the most abundant being Ascaris suum37. Six spots showed good quality spectra, but could not be identified. This appears to b...Continue Reading
References
Mar 1, 1991·Immunology Today·B S Polla
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·D B ElkinsR M Anderson
Aug 15, 1970·Nature·U K Laemmli
Jan 4, 2001·Current Biology : CB·F PianoK J Kemphues
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·A KarabinosK Weber
Sep 26, 2001·Parasitology Research·P UparanukrawA Dantrakool
Dec 5, 2002·International Journal for Parasitology·Naotoshi TsujiYasunobu Matsumoto
Mar 9, 2005·The FEBS Journal·Jochen KühnlEva Liebau
Feb 10, 2006·Development, Growth & Differentiation·Tadao InoueYasunori Yamaguchi
Apr 12, 2006·The Journal of Biological Chemistry·Ahmed A SayedDavid L Williams
Jun 9, 2006·International Journal for Parasitology·S Nikolaou, R B Gasser
Jul 3, 2010·Experimental Parasitology·Porntip LaummaunwaiWanchai Maleewong
Apr 9, 2011·Digestive Diseases and Sciences·Linlin DuXuchu Hu
May 21, 2011·Journal of Proteomics·Karina M RebelloAna G C Neves-Ferreira
Oct 25, 2011·Experimental Parasitology·Alessandra L MorassuttiCarlos Graeff-Teixeira
Sep 6, 2012·The American Journal of Tropical Medicine and Hygiene·Samuel Campista-LeónSylvia Páz Díaz-Camacho
Apr 23, 2013·Journal of Proteomics·Shu-Jian CuiFeng Liu
Sep 23, 2014·Acta Parasitologica·Ruofeng YanXiangrui Li
Citations
Nov 17, 2017·Journal of Helminthology·A MaX X Gan
Aug 16, 2018·Royal Society Open Science·Dawid SurmikBruce M Rothschild
Jan 29, 2017·Electrophoresis·Monica Di VenerePaolo Iadarola
Dec 11, 2020·Parasites & Vectors·Guo-Hua LiuChaoqun Yao
Jul 5, 2021·The Korean Journal of Parasitology·Yue WangXiao-Xian Gan