Proteomic analysis identifies insulin-like growth factor-binding protein-related protein-1 as a podocyte product.

American Journal of Physiology. Renal Physiology
Takayuki MatsumotoJeffrey B Kopp

Abstract

The podocyte secretory proteome may influence the phenotype of adjacent podocytes, endothelial cells, parietal epithelial cells, and tubular epithelial cells but has not been systematically characterized. We have initiated studies to characterize this proteome, with the goal of further understanding the podocyte cell biology. We cultured differentiated conditionally immortalized human podocytes and subjected the proteins in conditioned medium to mass spectrometry. At a false discovery rate of <3%, we identified 111 candidates from conditioned medium, including 44 proteins that have signal peptides or are described as secreted proteins in the UniProt database. As validation, we confirmed that one of these proteins, insulin-like growth factor-binding protein-related protein-1 (IGFBP-rP1), was expressed in mRNA and protein of cultured podocytes. In addition, transforming growth factor-β1 stimulation increased IGFBP-rP1 in conditioned medium. We analyzed IGFBP-rP1 glomerular expression in a mouse model of human immunodeficiency virus-associated nephropathy. IGFBP-rP1 was absent from podocytes of normal mice and was expressed in podocytes and pseudocrescents of transgenic mice, where it was coexpressed with desmin, a podocyte injury...Continue Reading

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Citations

Jul 30, 2010·American Journal of Physiology. Renal Physiology·Toru SakairiJeffrey B Kopp
May 3, 2014·International Journal of Nephrology·Mohsen NafarRoman A Zubarev
Oct 2, 2015·Journal of the American Society of Nephrology : JASN·Xiaoxin X WangMoshe Levi
Apr 25, 2015·Proteomics. Clinical Applications·Markus M RinschenTrairak Pisitkun
Jan 2, 2014·Kidney International·Börje S Haraldsson
Mar 5, 2011·Nihon Rinshō Men'eki Gakkai kaishi = Japanese journal of clinical immunology·Hiroshi Kajiyama
May 10, 2017·Frontiers in Immunology·Hua SuChun Zhang
Jul 6, 2017·Molecular Medicine Reports·Ying WuYanfeng Liu

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