Proteomic analysis of 17β-estradiol degradation by Stenotrophomonas maltophilia

Environmental Science & Technology
Zhongtian LiXu Li

Abstract

Microbial degradation plays a critical role in determining the environmental fate of steroid hormones, such as 17β-estradiol (E2). The molecular mechanisms governing the microbial transformation of E2 and its primary degradation intermediate, estrone (E1), are largely unknown. The objective of this study was to identify metabolism pathways that might be involved in microbial estrogen degradation. To achieve the objective, Stenotrophomonas maltophilia strain ZL1 was used as a model estrogen degrading bacterium and its protein expression level during E2/E1 degradation was studied using quantitative proteomics. During an E2 degradation experiment, strain ZL1 first converted E2 to E1 stoichiometrically. At 16 h E1 reached its peak concentration, and microbial growth started. At the same time, enzymes involved in certain catabolic and anabolic pathways were most highly expressed compared to the other time points tested. Among those enzymes, the ones involved in protein and lipid biosyntheses were observed to be particularly active. Based on the metabolite information from a previous study and the proteomic data from this study, we hypothesized that S. maltophilia strain ZL1 was able to convert E1 to amino acid tyrosine through ring ...Continue Reading

References

Jun 9, 1995·The Journal of Biological Chemistry·B González-Flecha, B Demple
Dec 11, 1999·Nucleic Acids Research·R L TatusovE V Koonin
Feb 22, 2000·The Journal of Biological Chemistry·L D NelsonM W Van Dyke
Mar 14, 2003·Nature·Ruedi Aebersold, Matthias Mann
Aug 20, 2003·Environmental Toxicology and Chemistry·Paul G TratnyekRené P Schwarzenbach
Oct 4, 2003·Environmental Science & Technology·Henrik AndersenThomas A Ternes
Aug 4, 2004·Applied Microbiology and Biotechnology·Stefanie WeberJuliane Hollender
Aug 7, 2004·Environmental Science & Technology·D Raj RamanGary S Sayler
Jul 2, 2005·Journal of Chromatography. a·Spyros GarbisMichael Fountoulakis
Nov 2, 2005·Chembiochem : a European Journal of Chemical Biology·Russell J CoxAndrea T Hadfield
Jan 13, 2006·Nature Chemical Biology·Shao-En Ong, Matthias Mann
Sep 27, 2006·Environmental Science & Technology·Shaily Mahendra, Lisa Alvarez-Cohen
Nov 4, 2006·Journal of Proteome Research·Bing ZhangNagiza F Samatova
Nov 14, 2006·Applied and Environmental Microbiology·R M MorrisR E Richardson
Dec 6, 2006·Environmental Science & Technology·Samir Kumar KhanalJ Van Leeuwent
Feb 22, 2007·Environmental Science & Technology·Chang-Ping YuKung-Hui Chu
May 29, 2007·Nucleic Acids Research·Yuki MoriyaMinoru Kanehisa
Jul 14, 2007·Environmental Science & Technology·Taewoo Yi, Willie F Harper
Sep 26, 2007·Proteomics·Natasha A Karp, Kathryn S Lilley
Apr 29, 2008·Nucleic Acids Research·Mark JohnsonThomas L Madden
Jul 12, 2008·The FEBS Journal·Rogerio MargisMarcia Margis-Pinheiro
Feb 28, 2009·Environmental Science & Technology·Mark J BenottiShane A Snyder
Dec 3, 2009·Proteomics·Thomas Schneider, Kathrin Riedel
Feb 4, 2010·Environmental Science & Technology·Adina S ChuangTimothy E Mattes
Sep 4, 2010·Environmental Science & Technology·Siqing XiaSlawomir W Hermanowicz
Mar 9, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Renu NandakumarGregor Grass
Apr 12, 2011·Applied and Environmental Microbiology·Lilach Iasur-KruhDror Minz

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Citations

Mar 23, 2013·Chemosphere·Chang-Ping YuKung-Hui Chu
Aug 21, 2013·Environmental Science & Technology·Shan LiuLiang-Ying He
May 28, 2013·Environmental Science & Technology·Michael L MashtareRonald F Turco
Mar 20, 2013·BioMed Research International·Elizabeth García-GómezIgnacio Camacho-Arroyo
Dec 20, 2015·Environmental Science & Technology·Henrik KjeldalJeppe Nielsen
Aug 24, 2016·Journal of Environmental Management·Jianlong Wang, Shizong Wang
Jan 27, 2017·International Journal of Environmental Research and Public Health·Shunyao LiXuezhu Zhu
Feb 12, 2014·Microbial Biotechnology·Yuping ZhangXu Li
Jan 30, 2020·Applied Microbiology and Biotechnology·Amit PratushZhong Hu
Dec 22, 2019·Journal of Hazardous Materials·Shunyao LiMichael Gatheru Waigi
Oct 18, 2013·The Journal of Physical Chemistry. a·Fumiya MorishimaTakayuki Ebata

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