Proteomic analysis of a clavata-like phenotype mutant in Brassica napus

Genetics and Molecular Biology
Keming ZhuXiaoli Tan

Abstract

Rapeseed is one of important oil crops in China. Better understanding of the regulation network of main agronomic traits of rapeseed could improve the yielding of rapeseed. In this study, we obtained an influrescence mutant that showed a fusion phenotype, similar with the Arabidopsis clavata-like phenotype, so we named the mutant as Bnclavata-like (Bnclv-like). Phenotype analysis illustrated that abnormal development of the inflorescence meristem (IM) led to the fused-inflorescence phenotype. At the stage of protein abundance, major regulators in metabolic processes, ROS metabolism, and cytoskeleton formation were seen to be altered in this mutant. These results not only revealed the relationship between biological processes and inflorescence meristem development, but also suggest bioengineering strategies for the improved breeding and production of Brassica napus.

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Methods Mentioned

BETA
electrophoresis
PCR
environmental stresses
nucleotide exchange

Software Mentioned

InterproScan
ImageMaster TM 2D platinum
Cytoscape
BLAST2GO
WEGO

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