Proteomic analysis of a ferric uptake regulator mutant of Helicobacter pylori: regulation of Helicobacter pylori gene expression by ferric uptake regulator and iron

Proteomics
Hyang Woo LeeNa Gyong Lee

Abstract

The ferric uptake regulator (Fur) protein is a Fe(2+)-dependent transcriptional repressor that binds to the Fur-box of bacterial promoters and down-regulates gene expression. In this study, to investigate global gene regulation by Fur in response to iron in Helicobacter pylori, a causative agent of human gastric diseases, we compared the proteome profiles of the H. pylori strain 26695 and its isogenic fur mutant grown under iron-rich and iron-depleted conditions. In total, 93 protein spots were found to be up- or down-regulated by more than 2-fold by either a fur mutation or iron-depletion. From these, 39 spots were identified by matrix-assisted laser desorption/ionization time of flight analysis to be 29 different proteins of diverse functions, including energy metabolism, transcription and translation, detoxification, biosynthesis of amino acids and nucleotides and production of the cell envelope. Expression of six proteins was found to be higher in the fur mutant than in the wild-type bacteria, indicating Fur-mediated repression. Eleven proteins were activated by Fur; five responded to iron and the others were not iron-responsive. The remaining 12 proteins were not under Fur-regulation but responded to iron in a positive or ...Continue Reading

Citations

Sep 19, 2009·The Journal of Microbiology·Wei QuJihui Jia
Jun 4, 2010·Antioxidants & Redox Signaling·Hitoshi TsugawaToshifumi Hibi
Oct 13, 2006·Infection and Immunity·Gregg S DavisHarry L T Mobley
Jun 22, 2006·Journal of Bacteriology·Alberto DanielliVincenzo Scarlato
Aug 29, 2006·PLoS Pathogens·David B FriedmanEric P Skaar
Jan 17, 2012·Journal of Clinical Biochemistry and Nutrition·Hidekazu SuzukiToshifumi Hibi
Oct 8, 2013·Journal of Bacteriology·Beth M CarpenterD Scott Merrell
Mar 9, 2006·Electrophoresis·Christina UwinsPhil Cash
Sep 10, 2008·Proteomics·Ansgar Poetsch, Dirk Wolters
Dec 8, 2010·Proteomics. Clinical Applications·Henry J WindleDermot P Kelleher
Oct 19, 2004·Trends in Microbiology·Arnoud H M van VlietJohannes G Kusters
Sep 23, 2014·Environmental Microbiology·Violeta C Sein-EchaluceMaría F Fillat
Jan 28, 2006·FEMS Microbiology Reviews·Karin van AmsterdamArie van der Ende
Aug 31, 2013·Gut Microbes·Timothy L Cover, Richard M Peek
Jun 6, 2009·FEMS Microbiology Letters·Young Wook ChoiNa Gyong Lee
Jul 20, 2016·Journal of Bacteriology·Stephanie L ServetasD Scott Merrell
Jan 19, 2008·Journal of Medical Microbiology·Chunhong ShaoJihui Jia
May 8, 2009·Biochemistry·Sylvia VitaleIsabelle Michaud-Soret

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.