Proteomic analysis of agar gel-entrapped Pseudomonas aeruginosa

Proteomics
Sébastien VilainThierry Jouenne

Abstract

We have compared the protein maps of agar-entrapped Pseudomonas aeruginosa cells to those of free counterparts grown in the presence or absence of the immobilized-cell gel support. Principal component analyses (PCAs) were used to interpret spot quantity variations observed on electropherograms obtained by two-dimensional gel electrophoresis. PCA of the data matrix (923 rows x 6 columns) in which spot density values were standardized horizontally extracted three principal components (PCs) with eigenvalues higher than 1, accounting together for 71.6% of the variability in the data. Principal component 1 (PC1) opposed free (F) and agar-entrapped (AE) cultures, with a low contribution of agar-released, free (ARF) cultures to PC1. Inversely, the contribution of ARF cultures to PC2 was high, opposing those of AE and F cultures. Component 3 was related to the duration of incubation. Only 10% of total proteins were upregulated in AE cells during the first 18 h of incubation, the number of underexpressed peptides balancing that of overexpressed ones. Downregulation clearly became the dominant tendency when the incubation time was extended to 48 h. These results demonstrate that AE and ARF bacteria are physiologically different from F or...Continue Reading

Citations

Sep 3, 2013·Journal of Proteome Research·Nichollas E ScottStuart J Cordwell
Apr 12, 2008·Journal of Biomedical Materials Research. Part a·Laetitia BénardGuy-Alain Junter
Jan 17, 2012·Proteomics·C Jayampath SeneviratneLakshman P Samaranayake
Nov 20, 2012·Electrophoresis·Janane RahbaniMazen Al-Ghoul
Dec 8, 2010·Proteomics. Clinical Applications·Nathan J Hare, Stuart J Cordwell
Dec 18, 2013·Journal of Proteomics·Rachel DuchesneSylvie Chevalier
Apr 14, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Claus MoserPeter Østrup Jensen
Aug 28, 2018·Expert Review of Proteomics·Charlotte GaviardJulie Hardouin
Oct 6, 2006·Antonie van Leeuwenhoek·Anthony ColletPatrick Di Martino
Oct 2, 2008·Journal of Proteome Research·Anthony ColletThierry Jouenne
Dec 13, 2005·Journal of Proteome Research·Laurent CoquetThierry Jouenne

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.