Proteomic analysis of brain tissue from an Alzheimer's disease mouse model by two-dimensional difference gel electrophoresis

Neurobiology of Aging
Daria SizovaAnita Diu-Hercend

Abstract

We used a beta-amyloid precursor protein (APP) transgenic (Tg) mouse model that displays some of the typical Alzheimer-associated pathological features to study the brain proteoma associated with amyloid plaque deposition. Two groups (male and female) of 14-month-old Tg mice were compared with their wild type littermates. We used differential 2D electrophoresis coupled with mass spectrometry to generate one of the first complete image of changes in brain protein expression occurring in this well-recognized model of Alzheimer's disease (AD). We identified 15 different proteins, which are significantly regulated in this pathology (p<0.05, > or =1.5-fold variation in expression comparing with the wild type samples). These comprise a number of proteins that were already known to be implicated in AD and neurodegeneration, as well as several proteins which relationship with AD had not been shown before. Identified proteins were grouped according to their biological key pathways. Results obtained are discussed in view of existing bibliographic data on human AD transcriptoma and proteoma.

References

Jan 1, 1994·Annual Review of Medicine·D L Price, S S Sisodia
Mar 1, 1996·Experimental Neurology·A AfaghA J Tenner
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·O I BuiakovaF L Margolis
Sep 18, 1997·Journal of Neuropathology and Experimental Neurology·M C IrizarryB T Hyman
Nov 5, 1997·Annals of the New York Academy of Sciences·H M WisniewskiJ Wegiel
Mar 18, 1999·The American Journal of Pathology·K YasojimaP L McGeer
Aug 12, 1999·Journal of Neural Transmission·M BiglK Eschrich
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·K R BalesS M Paul
Apr 25, 2000·Annual Review of Medicine·J N Buxbaum, C E Tagoe
Jul 14, 2000·The Journal of Biological Chemistry·J W ChenA B Fisher
Apr 6, 2001·The American Journal of Pathology·Y MatsuokaK Duff
May 16, 2001·Current Opinion in Lipidology·T Y ChangA Miyazaki
May 16, 2002·Neurobiology of Aging·C E TeunissenC De Bruijn
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yefim ManevichAron B Fisher
Oct 5, 2002·Disease Markers·W Hayes McDonald, John R Yates
Nov 5, 2002·Nature Reviews. Drug Discovery·Michael S Wolfe
Dec 25, 2002·The Journal of Biological Chemistry·Annett KochMichael Schrader
Apr 26, 2003·Acta Neurologica Scandinavica·G W RossL R White
May 7, 2003·The Journal of Biological Chemistry·Xiaosong WangBeverly Paigen
May 22, 2003·Progress in Neurobiology·Gert LubecMichael Fountoulakis
Jun 10, 2003·Annals of the New York Academy of Sciences·Johan van BeekPhilippe Gasque
Sep 3, 2003·Journal of Neurochemistry·D Allan ButterfieldAlessandra Castegna
Oct 22, 2003·Progress in Neurobiology·Yong Shen, Seppo Meri
Nov 26, 2003·Experimental Neurology·Véronique BlanchardLaurent Pradier
Mar 3, 2004·Molecular Interventions·K R BalesS M Paul
Apr 24, 2004·Annals of the New York Academy of Sciences·Bozho M Todorich, James R Connor
Jun 29, 2004·The Journal of Biological Chemistry·Lujian LiaoJunmin Peng
Jul 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Maria Isabel FonsecaAndrea J Tenner
Aug 28, 2004·Neuroscience Letters·Katja KanninenJari Koistinaho
Sep 21, 2004·Proteomics·Su Jeon ShinInhee Mook-Jung
Jul 29, 2005·The American Journal of Pathology·Samir Kumar-SinghChristine Van Broeckhoven

❮ Previous
Next ❯

Citations

Jun 30, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Christopher J MartyniukNancy D Denslow
Feb 5, 2010·Journal of Neuroinflammation·Lawrence L HorstmanAlireza Minagar
Jun 17, 2010·PloS One·Nicole SchonrockJürgen Götz
Dec 5, 2006·Biochimica Et Biophysica Acta·Xiongwei ZhuMark A Smith
Oct 21, 2014·PLoS Pathogens·Gilberto Hideo KaihamiRegina Lúcia Baldini
Mar 13, 2014·Journal of Proteome Research·Sravani MusunuriGanna Shevchenko
Feb 10, 2010·Journal of Psychopharmacology·Regina TaurinesJohannes Thome
Dec 15, 2015·Molecular & Cellular Proteomics : MCP·Guillermo Moya-AlvaradoFrancisca C Bronfman
Nov 1, 2007·Proteomics. Clinical Applications·Keith Vosseller
May 1, 2008·Proteomics. Clinical Applications·Nicolas BousetteThomas Kislinger
Mar 10, 2009·Fundamental & Clinical Pharmacology·François ReichardtJean-Hervé Lignot
Feb 2, 2010·Journal of Neurosurgical Anesthesiology·Armin KalenkaRobert E Feldmann
Jul 9, 2020·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Luciana Patricia Fernandes AbbadeBenedito Barraviera
Feb 26, 2016·Molecular Neurobiology·Katalin VölgyiKatalin Adrienna Kékesi
May 21, 2021·Molecular Neurobiology·Jiangfeng LiaoJing Zhang
Mar 14, 2008·Neurobiology of Aging·Mark J WestJens Ledet Jensen
Aug 16, 2008·Ageing Research Reviews·Renã A SowellD Allan Butterfield

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes & Neurodegeneration

Astrocytes are important for the health and function of the central nervous system. When these cells stop functioning properly, either through gain of function or loss of homeostatic controls, neurodegenerative diseases can occur. Here is the latest research on astrocytes and neurodegeneration.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.

Alzheimer's Disease: Animal Models

Alzheimer's disease is a neurodegenerative disease which can be studied using various experimental systems. This feed focuses on animal models used for Alzheimer's disease research.

Alzheimer's Disease: APOE

Apolipoprotein E (APOE) polymorphic alleles are major genetic risk factors for Alzheimer's disease. Discover the latest research on APOE and other genetic determinants of Alzheimer's disease here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Astrocytes & Amyloid

Astrocytes are thought to play a role in amyloid production and have been implicated in neurodegenerative diseases such as Alzheimer’s disease. Here is the latest research on the relationship between astrocytes and amyloid.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

Alzheimer's Disease: Microglia

Microglia are a type of glial cell found throughout the brain and spinal cord. Microglia have been found to be associated with Alzheimer's disease development and progression. Here are the latest discoveries pertaining to Alzheimer's disease and microglia.