Proteomic analysis of calcified abdominal and thoracic aortic aneurysms

International Journal of Molecular Medicine
Ken-Ichi MatsumotoTeiji Oda

Abstract

Aortic aneurysm is a complex multifactorial disease with genetic and environmental risk factors. It is often accompanied by aortic calcification. Here, to uncover proteins that are significantly changed in calcified abdominal aortic aneurysms (CAAs) and calcified thoracic aortic aneurysms (CTAs) compared with those in adjacent normal aorta tissues, comprehensive analysis of differentially expressed proteins in their tissues was performed by a quantitative proteomic approach with iTRAQ labeling in combination with nanoLC-MALDI-TOF/TOF-MS/MS followed by ProteinPilot analysis. The proteomic analysis revealed 138 and 134 proteins differentially expressed in CAAs and CTAs in contrast to neighboring normal aorta tissues with high confidence, respectively. Significantly increased expression (≥1.3-fold) was found in 41 and 28 proteins, whereas decreased expression (<0.77-fold) was found in 4 and 60 proteins in CAAs and CTAs, respectively. Among them, we identified already known proteins involved in aneurysm formation and vascular calcification, such as type I and III collagen, matrix Gla protein, and α-2-HS-glycoprotein in CAAs and fibrinogen α, β and γ chains and α-2-HS-glycoprotein in CTAs with increased expression and mimecan in CAA...Continue Reading

References

Jan 1, 1992·Circulation·D ReedT Hayashi
Feb 1, 1987·Proceedings of the Society for Experimental Biology and Medicine·M A DubickC L Keen
Aug 1, 1994·Current Opinion in Lipidology·R M Krauss, Y A Kesäniemi
Mar 1, 1997·Cardiovascular Research·L T Jensen, N B Høst
Dec 10, 1999·Cardiology Clinics·M A CoadyJ A Elefteriades
Jan 24, 2002·Nature·Tomoyuki NakamuraKenneth R Chien
Feb 12, 2002·Bioinformatics·Alexander SturnZlatko Trajanoski
Jul 18, 2002·Atherosclerosis·Anne TailleuxVéronique Clavey
Mar 14, 2003·Biochimica Et Biophysica Acta·Simona StanEmile Levy
Sep 24, 2004·Molecular & Cellular Proteomics : MCP·Philip L RossDarryl J Pappin
Feb 26, 2005·Clinical Nephrology·C M Shanahan
Apr 14, 2005·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kirsty GreenowDipak P Ramji
Sep 16, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Jonathan GolledgePaul Norman
Jan 16, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Michael L McCormickNeal L Weintraub
Feb 27, 2007·The American Journal of Pathology·Hazem Abdul-HussienJan H N Lindeman
Jan 16, 2008·Circulation·Helena KuivaniemiM David Tilson
Feb 9, 2008·World Journal of Surgery·Suman AnnambhotlaChangyi Chen
Feb 23, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Ziad TouatGuillaume Jondeau
May 21, 2008·Atherosclerosis·Jeffrey W StephensSteve E Humphries
Nov 26, 2008·The Journal of Thoracic and Cardiovascular Surgery·Jean Marie RuddyJohn S Ikonomidis
Mar 6, 2009·Journal of Atherosclerosis and Thrombosis·Akio Kawakami, Masayuki Yoshida
Aug 1, 2008·Current Cardiology Reviews·Gabriel RusanescuElena Aikawa
May 11, 2010·The American Journal of Cardiology·Jonathan GolledgePaul E Norman
Nov 3, 2010·Cardiovascular Research·Jean-Baptiste MichelUNKNOWN FAD EU consortium
May 20, 2011·Molecular & Cellular Proteomics : MCP·Athanasios DidangelosManuel Mayr
Jun 21, 2011·Journal of Nephrology·Raja RezgZiad A Massy
Oct 1, 2011·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Jessica Kendrick, Michel Chonchol

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Citations

Jun 22, 2013·Proteomics. Clinical Applications·Zongming FuJennifer Van Eyk
Mar 6, 2018·Journal of Atherosclerosis and Thrombosis·Toshiki SekiYukihiko Momiyama
Jul 14, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Sarah J ParkerJennifer E Van Eyk
Jul 25, 2015·Journal of the American Heart Association·Xiaosong GuJianchang Chen
Jan 18, 2014·International Journal of Molecular Medicine·Ken-ichi MatsumotoTeiji Oda
Dec 10, 2014·Anatomia, histologia, embryologia·M Kobayashi-MiuraY Fujita
Apr 16, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sophie DeckxAnna-Pia Papageorgiou
May 19, 2019·Science China. Life Sciences·Fanshuang ZhangYouhe Gao
May 31, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Ploingarm PetsophonsakulLeon Schurgers
Jan 6, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yongjiang QianZhongqun Wang
Apr 13, 2021·Expert Review of Proteomics·Jianqiang WuYuehong Zheng
Sep 30, 2014·Journal of Proteome Research·Muge SerhatliAhmet Tarik Baykal

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