Proteomic analysis of canine oral tumor tissues using MALDI-TOF mass spectrometry and in-gel digestion coupled with mass spectrometry (GeLC MS/MS) approaches

PloS One
Sirinun PisamaiGunnaporn Suriyaphol

Abstract

Oral tumors, including highly invasive and metastatic oral melanoma (OM), non-tonsillar oral squamous cell carcinoma (OSCC) and benign tumors (BN), are common neoplasms in dogs. Although these tumors behave differently, limited data of their protein expression profiles have been exhibited, particularly at the proteome level. The present study aimed to i.) characterize peptide-mass fingerprints (PMFs) and identify potential protein candidates of OM, OSCC, BN and normal control subjects, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS), ii.) identify potential protein candidates associated with the diseases, using in-gel digestion coupled with mass spectrometric analysis (GeLC-MS/MS) and iii.) search for relationships between chemotherapy drugs and disease-perturbed proteins. A distinct cluster of each sample group and unique PMFs with identified protein candidates were revealed. The unique peptide fragment at 2,274 Da of sacsin molecular chaperone (SACS) was observed in early-stage OM whereas the fragment at 1,958 Da of sodium voltage-gated channel alpha subunit 10 (SCN10A) was presented in early- and late-stage OM. The...Continue Reading

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Citations

May 13, 2020·Oncogene·Hossein TabatabaeianYoon Pin Lim
Aug 20, 2021·PloS One·Sekkarin PloypetchGunnaporn Suriyaphol

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Methods Mentioned

BETA
PCA
electrophoresis
nucleotide exchange
biopsy
two-hybrid
affinity purification

Software Mentioned

DeCyder MS differential
DeCyder
flexAnalysis
DeCyder MS
PANTHER
Stitch
ClinProTools
CompassXport
MASCOT
Multiexperiment Viewer ( MeV )

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