Proteomic analysis of CD4+ T-lymphocytes in patients with asthma between typical therapy (controlled) and no typical therapy (uncontrolled) level

Human & Experimental Toxicology
Yang-Ching KoWen-Pin Wu

Abstract

T-lymphocyte (T-LC)-derived cytokines have been implicated in asthmatic pathogenesis. Proteomic technology is now widely accepted as a complementary technology to genetic profiling. We investigated the changes of proteins in T-LC of asthmatic patients from the no typical therapy (uncontrolled) to typical therapy (controlled) level by using standard proteome technology. The proteins of CD4+ T-LC were isolated from the whole blood of six asthmatic patients from uncontrolled to controlled levels over 3 months. Two-dimensional polyacrylamide gel electrophoresis was performed and coomassie blue stained protein spots were comparatively analyzed by using an image analyzer. Some differentially expressed spots were identified by liquid chromatography/mass spectrometry and database search. Our results showed that 13 proteins showed different expression. Six protein spots in the CD4+ T-LC of the uncontrolled asthmatic patients were increased and 7 spots were decreased compared to those of the controlled subjects. In conclusion, the proteomic examination of the CD4+ T-LC revealed some differentially expressed proteins in the uncontrolled and controlled asthmatic patients. The possibility of using the differentially expressed proteins as im...Continue Reading

References

Jan 30, 1992·The New England Journal of Medicine·D S RobinsonA B Kay
May 1, 1991·The American Review of Respiratory Disease·A Laitinen, L A Laitinen
Oct 11, 1990·The New England Journal of Medicine·J BousquetP Godard
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S P BlattiM J Getz
Jan 1, 1982·Methods in Enzymology·L B Smillie
Feb 26, 1998·American Journal of Respiratory and Critical Care Medicine·A M VignolaJ Bousquet
Apr 30, 1999·Allergy·Y AronF Favatier
Oct 7, 2000·Respirology : Official Journal of the Asian Pacific Society of Respirology·W Tong, W Luo
Feb 15, 2001·The New England Journal of Medicine·W W Busse, R F Lemanske
Aug 11, 2001·Cellular and Molecular Life Sciences : CMLS·V Pancholi
Aug 29, 2003·Cellular and Molecular Life Sciences : CMLS·M F Sánchez-FontR Gonzàlez-Duarte
Jan 1, 1960·Journal of Clinical Pathology·M S DUNNILL
May 14, 2004·American Journal of Physiology. Endocrinology and Metabolism·K Sreekumaran NairSreekumar Raghavakaimal
May 21, 2004·American Journal of Respiratory and Critical Care Medicine·Ignacio FajardoGunnar Pejler
Dec 4, 2004·The Journal of Allergy and Clinical Immunology·Jae-Ouk KimChang-Yuil Kang
Nov 9, 2005·The Journal of Allergy and Clinical Immunology·Christine M Seroogy, James E Gern
Aug 8, 2006·The Journal of Allergy and Clinical Immunology·Dong-Ho NahmHae-Sim Park
Aug 18, 2006·Nucleic Acids Research·Tuuli VälinevaOlli Silvennoinen
Jan 2, 2008·The European Respiratory Journal·E D BatemanH J Zar

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Citations

Jan 9, 2014·Journal of Translational Medicine·Jiebai ZhouXiangdong Wang
Sep 5, 2014·Proteomics. Clinical Applications·Rossana RossiPierluigi Mauri
Jul 12, 2016·Expert Review of Respiratory Medicine·Diego BagnascoGiorgio Walter Canonica
Nov 11, 2014·Journal of Proteome Research·Luis M TeranThomas Franz

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