Proteomic analysis of cellular protein expression profiles in response to grass carp reovirus infection

Fish & Shellfish Immunology
Dan XuLiqun Lu

Abstract

Grass carp (Ctenopharyngodon idella) hemorrhagic disease, caused by grass carp reovirus (GCRV), is emerging as a serious problem in grass carp aquaculture. To better understand the molecular responses to GCRV infection, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization tandem mass spectroscopy were performed to investigate altered proteins in C. idella kidney (CIK) cells. Differentially expressed proteins in mock infected CIK cells and GCRV-infected CIK cells were compared. Twenty-three differentially expressed spots were identified (22 upregulated spots and 1 downregulated spot), which included cytoskeleton proteins, macromolecular biosynthesis-associated proteins, stress response proteins, signal transduction proteins, energy metabolism-associated proteins and ubiquitin proteasome pathway-associated proteins. Moreover, 10 of the corresponding genes of the differentially expressed proteins were quantified by real-time reverse transcription polymerase chain reaction to examine their transcriptional profiles. The T cell internal antigen 1 (TIA1) and Ras-GTPase-activating SH3-domain-binding protein1 (G3BP1) of the cellular stress granule pathway from grass carp C. idella (designated as CiTIA1 ...Continue Reading

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Citations

Nov 30, 2015·Archives of Virology·Jia LiuQi-Ya Zhang
Sep 15, 2017·International Journal of Molecular Sciences·Libo HeAnd Yaping Wang
Apr 28, 2017·Journal of Virology·Long-Feng LuYong-An Zhang
Apr 13, 2018·International Journal of Molecular Sciences·Lv XiongYaping Wang
Jan 13, 2021·Animals : an Open Access Journal From MDPI·Márcio MoreiraPedro Rodrigues

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