Proteomic analysis of cerebrospinal fluid from children with central nervous system tumors identifies candidate proteins relating to tumor metastatic spread

Oncotarget
Filippo SpreaficoMaura Massimino

Abstract

Central nervous system (CNS) tumors are the most common solid tumors in childhood. Since the sensitivity of combined cerebrospinal fluid (CSF) cytology and radiological neuroimaging in detecting meningeal metastases remains relatively low, we sought to characterize the CSF proteome of patients with CSF tumors to identify biomarkers predictive of metastatic spread. CSF samples from 27 children with brain tumors and 13 controls (extra-CNS non-Hodgkin lymphoma) were processed using core-shell hydrogel nanoparticles, and analyzed with reverse-phase liquid chromatography/electrospray tandem mass spectrometry (LC-MS/MS). Candidate proteins were identified with Fisher's exact test and/or a univariate logistic regression model. Reverse phase protein array (RPPA), Western blot (WB), and ELISA were used in the training set and in an independent set of CFS samples (60 cases, 14 controls) to validate our discovery findings. Among the 558 non-redundant proteins identified by LC-MS/MS, 147 were missing from the CSF database at http://www.biosino.org. Fourteen of the 26 final top-candidate proteins were chosen for validation with WB, RPPA and ELISA methods. Six proteins (type 1 collagen, insulin-like growth factor binding protein 4, procollag...Continue Reading

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Citations

Jan 19, 2018·International Journal of Environmental Research and Public Health·Xin LiChunyan Zhao
Nov 14, 2019·Proteomics. Clinical Applications·Masaki SuzukiNaohiko Koshikawa
Aug 9, 2020·Journal of Neuropathology and Experimental Neurology·Karen TangMatija Snuderl
Dec 28, 2018·NPJ Precision Oncology·Erin R BonnerJavad Nazarian
Jun 4, 2020·Clinical Proteomics·Andrew MacklinThomas Kislinger
Apr 14, 2020·Advances in Radiation Oncology·Matthew S DietzJerry J Jaboin

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Methods Mentioned

BETA
Protein ANalysis
protein array
enzyme-linked immunosorbent assay
ELISA
biopsy

Software Mentioned

Scaffold
Proteome
Panther
BodyFluid
Quantity One
Protein ANalysis THrough Evolutionary Relationships ( PANTHER )
MicroVigene

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