Proteomic analysis of chick retina during early recovery from lens‑induced myopia

Molecular Medicine Reports
Yun Yun ZhouChi Ho To

Abstract

Myopia development has been extensively studied from different perspectives. Myopia recovery is also considered important for understanding the development of myopia. However, despite several previous studies, retinal proteomics during recovery from myopia is still relatively unknown. Therefore, the aim of the present study was to investigate the changes in protein profiles of chicken retinas during early recovery from lens‑induced myopia to evaluate the signals involved in the adjustment of this refractive disorder. Three‑day old chickens wore glasses for 7 days (‑10D lens over the right eye and a plano lens as control over the left eye), followed by 24 h without lenses. Protein expression in the retina was measured by two‑dimensional fluorescence difference gel electrophoresis (2D‑DIGE). Pro‑Q Diamond phosphoprotein staining 2D gel electrophoresis was used to analyze phosphoprotein profiles. Protein spots with significant differences (P<0.05) were analyzed by mass spectrometry. The minus lens‑treated eye became myopic, however following 24 h recovery, less myopia was observed. 2D‑DIGE proteomic analysis demonstrated that three identified protein spots were upregulated at least 1.2‑fold in myopic recovery retinas compared with...Continue Reading

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Citations

Nov 1, 2020·Journal of Clinical Medicine·Emil Tomasz GrochowskiDiana Anna Dmuchowska

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Methods Mentioned

BETA
glycosylation
electrophoresis
PCR

Software Mentioned

DeCyder Differential Analysis
MASCOT engine
Image Master Platinum

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