Abstract
Sanqi, the root of Panax notoginseng, is a popularly used traditional Chinese medicine with cardiovascular effects. Notoginsengnosides (NG) isolated from Sanqi could inhibit ADP-induced platelet aggregation of rat washed platelets. To identify the possible target proteins of NG in platelets, two-dimensional gel electrophoresis (2-DE)-based comparative proteomics was performed and proteins altered in expressional level after NG treatment were identified by MALDI-TOF MS/MS. Treatment of 200 microg/ml NG caused regulation of the levels of 12 proteins, which play important roles in platelet activation, oxidative stress and cytoskeleton. In the NG-treated platelets, there were increase in the levels of growth factor receptor-bound protein 2 (Grb2), thrombospondin 1, tubulin alpha 6 and decrease in the levels of thioredoxin, Cu-Zn superoxide dismutase, DJ-1 protein, peroxiredoxin 3, thioredoxin-like protein 2, ribonuclease inhibitor, potassium channel subfamily V member 2, myosin regulatory light chain 9 and laminin receptor 1. The change in the levels of these proteins caused by NG treatment might contribute to the inhibitive effect of NG on platelet aggregation. Furthermore, analysis of the reactive oxygen species (ROS) level indic...Continue Reading
References
Aug 7, 1992·Cell·E J LowensteinJ Schlessinger
Sep 1, 1990·The Journal of Physiology·M P Mahaut-SmithS O Sage
Apr 1, 1990·Planta medica·S C KuoT S Wu
Dec 1, 1988·The Journal of Pharmacy and Pharmacology·Y KimuraS Arichi
Sep 1, 1995·The International Journal of Biochemistry & Cell Biology·D NadanoK Kishi
Nov 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·C LegrandA Panet
Mar 1, 1996·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·S Rabhi-SabileC Legrand
Sep 18, 1999·Free Radical Biology & Medicine·H Wang, J A Joseph
Jan 15, 2000·The Journal of Biological Chemistry·S WitteA Altman
Apr 20, 2002·The Biochemical Journal·Abdelhafid SaciChristilla Bachelot-Loza
Feb 6, 2003·The Journal of Biological Chemistry·Christine M SadekAntonio Miranda-Vizuete
Mar 5, 2003·Journal of Neurochemistry·Hong Wang, Georg Reiser
Oct 6, 2005·Blood·Arnaud BonnefoyMarc F Hoylaerts
Apr 25, 2006·Free Radical Biology & Medicine·Hong-Sheng Zhang, Sheng-Qi Wang
May 31, 2006·Biochemistry. Biokhimii︠a︡·Shuo Wang, Haiping Li
Jul 29, 2006·The Journal of Pharmacy and Pharmacology·T B Ng
Aug 24, 2006·Blood·Dmitri V GnatenkoWadie F Bahou
Oct 25, 2006·Journal of Thrombosis and Haemostasis : JTH·L SunA K Rao
Oct 25, 2006·The Journal of Cell Biology·Sudit S MukhopadhyaySharon E Plon
Mar 7, 2007·Seminars in Thrombosis and Hemostasis·Stephan Lindemann, Meinrad Gawaz
May 31, 2007·Neurological Research·Yuhui WangZhengrong Wang
Jul 28, 2007·Life Sciences·Kai SunJing-Yan Han
Sep 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eva Andres-MateosValina L Dawson
Sep 25, 2007·Blood·Jeff S IsenbergDavid D Roberts
Jan 2, 2008·Molecular & Cellular Proteomics : MCP·Qing-Xi YueDe-An Guo
Jan 29, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Chao MaDe-An Guo
May 7, 2008·The American Journal of Chinese Medicine·Yan YaoDe-An Guo
Citations
Nov 27, 2010·Chinese Journal of Integrative Medicine·Hui QiangKun-zheng Wang
Mar 22, 2013·Omics : a Journal of Integrative Biology·Aihua ZhangXijun Wang
Feb 10, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Hui QiangLi-hong Fan
Mar 7, 2014·Experimental Gerontology·Yu-Jin WonSi-Kwan Kim
Feb 22, 2014·Expert Opinion on Investigational Drugs·Jingjing LiuChunming Wang
Dec 25, 2012·Journal of Pharmaceutical and Biomedical Analysis·Hui SunXijun Wang
Oct 22, 2011·Blood Cells, Molecules & Diseases·Jin-Yu HuYing-Wei Wang
Oct 12, 2013·Science China. Life Sciences·Yue LiuKeji Chen
Sep 23, 2011·Biochemical Society Transactions·Xuan Liu, De-An Guo
Apr 14, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Hui QiangChen Zhang
Aug 26, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaochen YangJie Wang
May 8, 2016·Journal of Ethnopharmacology·Ting WangZhuju Wang
Nov 12, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Meina ShiWeimin Yang
Sep 23, 2016·Pharmaceutical Biology·Chun-Hong LiFeng-Qing Yang
Apr 24, 2020·Chinese Medicine·Shi-Jun YinFeng-Qing Yang
Nov 2, 2017·Frontiers in Pharmacology·Lian DuanJie Wang
May 22, 2018·Frontiers in Physiology·Wang LiuXuan Liu
Jan 15, 2019·Chinese Medicine·Yi-Yao YangJian-Li Gao
Feb 25, 2014·Molecular Biology Reports·Ji LiuXiaoming Deng
Jan 21, 2021·Chinese Medicine·Zhi-Yi XuYu-Jiang Fan
Mar 6, 2019·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Lu Jia-XiGuo De-An
Apr 17, 2021·Blood Cells, Molecules & Diseases·Yu-Wei WanXuan Liu