Proteomic analysis of differential protein expression in rat platelets treated with notoginsengnosides

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Yan YaoDe-An Guo

Abstract

Sanqi, the root of Panax notoginseng, is a popularly used traditional Chinese medicine with cardiovascular effects. Notoginsengnosides (NG) isolated from Sanqi could inhibit ADP-induced platelet aggregation of rat washed platelets. To identify the possible target proteins of NG in platelets, two-dimensional gel electrophoresis (2-DE)-based comparative proteomics was performed and proteins altered in expressional level after NG treatment were identified by MALDI-TOF MS/MS. Treatment of 200 microg/ml NG caused regulation of the levels of 12 proteins, which play important roles in platelet activation, oxidative stress and cytoskeleton. In the NG-treated platelets, there were increase in the levels of growth factor receptor-bound protein 2 (Grb2), thrombospondin 1, tubulin alpha 6 and decrease in the levels of thioredoxin, Cu-Zn superoxide dismutase, DJ-1 protein, peroxiredoxin 3, thioredoxin-like protein 2, ribonuclease inhibitor, potassium channel subfamily V member 2, myosin regulatory light chain 9 and laminin receptor 1. The change in the levels of these proteins caused by NG treatment might contribute to the inhibitive effect of NG on platelet aggregation. Furthermore, analysis of the reactive oxygen species (ROS) level indic...Continue Reading

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Citations

Feb 10, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Hui QiangLi-hong Fan
Feb 22, 2014·Expert Opinion on Investigational Drugs·Jingjing LiuChunming Wang
Oct 22, 2011·Blood Cells, Molecules & Diseases·Jin-Yu HuYing-Wei Wang
Sep 23, 2011·Biochemical Society Transactions·Xuan Liu, De-An Guo
Apr 14, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Hui QiangChen Zhang
Aug 26, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Xiaochen YangJie Wang
Feb 25, 2014·Molecular Biology Reports·Ji LiuXiaoming Deng
Apr 17, 2021·Blood Cells, Molecules & Diseases·Yu-Wei WanXuan Liu

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