Proteomic analysis of differentially expressed proteins between the male and female worm of Schistosoma japonicum after pairing

Proteomics
Guo-Feng ChengYou-Min Cai

Abstract

Identification of differentially expressed proteins between the male and female worm of Schistosoma japonicum may provide new insights into the development of schistosomes, especially the molecular mechanism of female worm maturation induced by the male worm after pairing. Comparative two-dimensional gel electrophoresis (2-DE) and mass spectrometry were employed to separate and identify differentially expressed proteins between the male and female worm after pairing. Soluble and hydrophobic proteins from egg, schistosomulum (14 days), and female and male worms at adult stage (42 days) were separated by a sequential extraction method followed by 2-DE and 2-DE images were constructed. There were 1016 +/- 67, 1808 +/- 89, 1142 +/- 45 and 1288 +/- 32 spots detected for soluble proteins and 1425 +/- 108, 952 +/- 59, 847 +/- 75 and 965 +/- 69 spots for hydrophobic proteins from egg, schistosomulum, and adult stage female and male worms, respectively. The differentially and uniquely expressed proteins from male and female worms after pairing (42 days) include 41 +/- 4 and 23 +/- 2 unique spots for soluble proteins, and 11 +/- 1 and 26 +/- 3 unique spots for hydrophobic proteins, respectively. Matrix-assisted laser desorption/ionizatio...Continue Reading

References

Jan 1, 1989·Experimental Parasitology·P T LoVerdeL A Bobek
Dec 1, 1985·Parasitology·J E Den Hollander, D A Erasmus
Aug 1, 1982·Molecular and Biochemical Parasitology·A J SimpsonT F McCutchan
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Apr 3, 2001·Trends in Parasitology·P D AshtonR A Wilson
May 3, 2001·Electrophoresis·W M LauberJ P Malone
Oct 22, 2002·Molecular and Biochemical Parasitology·Javier E GirardiniEsteban Serra
May 14, 2003·Molecular and Biochemical Parasitology·Jon P BoyleTimothy P Yoshino
Sep 16, 2003·Nature Genetics·Sergio Verjovski-AlmeidaEmmanuel Dias-Neto
Nov 1, 1991·Parasitology Today·P T Loverde, L Chen

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Citations

Feb 18, 2010·Parasitology Research·Jinbiao PengJiaojiao Lin
Jul 11, 2012·Parasitology·Peter M BrophyRussell M Morphew
Jul 28, 2009·Annual Review of Genomics and Human Genetics·Ze-Guang HanZhu Chen
Nov 11, 2009·PLoS Neglected Tropical Diseases·Marcela G DrummondGlória R Franco
Sep 27, 2013·PLoS Neglected Tropical Diseases·Antonio MarcoMatthew Ronshaugen
Mar 22, 2014·PLoS Neglected Tropical Diseases·Fernanda LudolfRosiane A Silva-Pereira
Aug 29, 2012·The Journal of Parasitology·Yang HongJiaojiao Lin
Oct 1, 2007·Expert Opinion on Drug Discovery·Matthew BerrimanJames P McCarter
May 12, 2009·Molecular and Biochemical Parasitology·Henrique K RofattoLeonardo P Farias
May 12, 2009·Drug Discovery Today·Ricardo DeMarco, Sergio Verjovski-Almeida
Oct 23, 2008·Parasitology International·Ekhlas Hamed Abdel-HafeezKenji Hirayama
Nov 14, 2006·Trends in Parasitology·R Alan WilsonAlasdair Ivens
Oct 17, 2009·Proteomics·Ingrid MillerElisabetta Gianazza
Jun 10, 2008·Acta Tropica·Sergio Verjovski-Almeida, Ricardo DeMarco
Mar 21, 2006·International Journal for Parasitology·Jaap J Van HellemondAloysius G M Tielens
Jul 13, 2007·Experimental Parasitology·Guilherme Oliveira
Jun 20, 2007·Experimental Parasitology·Jaap J van HellemondAloysius G M Tielens
Mar 6, 2007·Trends in Parasitology·Cornelis H HokkeKarl F Hoffmann
Jan 13, 2015·Parasitology Research·Adriana S A PereiraMaria E C Chaves
Jul 26, 2018·PLoS Neglected Tropical Diseases·Juntao LiuGuofeng Cheng
Jun 29, 2010·Parasitology Research·Jing ChenGuofeng Cheng

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