Proteomic analysis of differentially expressed proteins in Penaeus monodon hemocytes after Vibrio harveyi infection.

Proteome Science
Kunlaya SomboonwiwatAnchalee Tassanakajon

Abstract

Viral and bacterial diseases can cause mass mortalities in commercial shrimp aquaculture. In contrast to studies on the antiviral response, the responses of shrimps to bacterial infections by high throughput techniques have been reported only at the transcriptional level and not at the translational level. In this study, a proteomic analysis of shrimp hemocytes to identify differentially expressed proteins in response to a luminous bacterium Vibrio harveyi was evaluated for its feasibility and is reported for the first time. The two-dimensional gel electrophoresis (2-DE) patterns of the hemocyte proteins from the unchallenged and V. harveyi challenged shrimp, Penaeus monodon, at 24 and 48 h post infection were compared. From this, 27 differentially expressed protein spots, and a further 12 weakly to non-differentially regulated control spots, were selected for further analyses by the LC-ESI-MS/MS. The 21 differentially expressed proteins that could be identified by homologous annotation were comprised of proteins that are directly involved in the host defense responses, such as hemocyanin, prophenoloxidase, serine proteinase-like protein, heat shock protein 90 and alpha-2-macroglobulin, and those involved in signal transduction...Continue Reading

References

Jan 1, 1977·Scandinavian Journal of Immunology·G Kaplan
Mar 15, 1996·Science·D Görlich, I W Mattaj
Mar 20, 2004·Pharmacology & Therapeutics·Amere Subbarao Sreedhar, Peter Csermely
Apr 9, 2004·FEBS Letters·Amere Subbarao SreedharYu-Fei Shen
Jun 18, 2004·Immunological Reviews·Lage Cerenius, Kenneth Söderhäll
Dec 9, 2004·Diseases of Aquatic Organisms·Premruethai SupungulAnchalee Tassanakajon
Jun 1, 2005·Biochimica Et Biophysica Acta·Maria D MavrouliVassilis J Marmaras
Aug 2, 2005·Journal of Biotechnology·Moltira TonganuntKenichi Fujise
Oct 14, 2005·Bioscience Reports·Ida Chiara Guerrera, Oliver Kleiner
Feb 10, 2006·Journal of Biochemistry and Molecular Biology·Kunlaya SomboonwiwatAnchalee Tassanakajon
May 16, 2006·Immunobiology·Pikul JiravanichpaisalKenneth Söderhäll
Dec 26, 2006·Developmental and Comparative Immunology·Hao-Ching WangChu-Fang Lo
Apr 25, 2007·Developmental and Comparative Immunology·Thanawat SriphaijitSaengchan Senapin
Jun 15, 2007·Physiological Genomics·Enrique de la VegaBernard M Degnan
Sep 9, 2008·Developmental and Comparative Immunology·Hua-Shui AiJian-Guo He
Oct 7, 2008·Developmental and Comparative Immunology·Piti AmparyupAnchalee Tassanakajon
Jan 27, 2009·Journal of Proteome Research·Weifeng LiuXiaobo Zhang
Mar 31, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Rungrat JitvaropasAnchalee Tassanakajon
Jun 22, 2010·Developmental and Comparative Immunology·Wanilada RungrassameeNitsara Karoonuthaisiri

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Citations

Dec 4, 2013·Journal of Biosciences·Vinayak BiradarDeepti Deobagkar
Oct 20, 2012·PloS One·Vorrapon ChaikeeratisakAnchalee Tassanakajon
Oct 13, 2012·Fish & Shellfish Immunology·Anchalee TassanakajonSureerat Tang
Mar 13, 2012·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Witchulada TalakhunPiamsak Menasveta
Apr 14, 2012·Journal of Proteomics·Pedro M RodriguesFlemming Jessen
Jun 20, 2017·Developmental and Comparative Immunology·Zhuang XueLinsheng Song
Jun 13, 2017·Fish & Shellfish Immunology·Baojian ZhuLiangli Yang
Aug 12, 2014·Applied and Environmental Microbiology·Marion CardinaudChristine Paillard
Feb 4, 2012·Molecular Biology Reports·Vorrapon ChaikeeratisakAnchalee Tassanakajon
Nov 22, 2011·Molecular Biology Reports·Chengwen SongShuangyan Wang
Feb 17, 2021·Scientific Reports·Pakpoom BoonchuenKunlaya Somboonwiwat

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Methods Mentioned

BETA
electrophoresis
GTPases
GTPase
PCR
X-ray

Software Mentioned

MASCOT
ImageMaster 2D Platinum

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