Proteomic analysis of elicitation of downy mildew disease resistance in pearl millet by seed priming with β-aminobutyric acid and Pseudomonas fluorescens

Journal of Proteomics
Chandra Pal AnupKukkundoor Ramachandra Kini

Abstract

Downy mildew is one of the severe diseases of pearl millet, globally affecting its commercial production. Priming of seeds of a susceptible cultivar of pearl millet with β-aminobutyric acid (BABA) and Pseudomonas fluorescens has reduced the downy mildew disease incidence level under field studies. In the current study, proteomic approach was used to elucidate the poorly studied resistance mechanism in these elicitor primed pearl millet seeds in response to Sclerospora graminicola infection. 2DE-MS/MS based proteomic approach revealed that majority of the 63 differentially accumulated (p≤0.05) proteins associated with energy and metabolism followed by stress and defense category. Multivariate statistics disclosed that infection caused by the pathogen rather than elicitor treatment had a major influence on the dynamics of protein abundance. Mechanism of priming mediated by BABA and P. fluorescens were different from each other as evident by the protein abundance profile of hierarchical clustering analysis. Over-representation of proteins pertaining to glucose metabolism suggests that seed priming ensures plant protection against disease without compromising its normal growth and development. In addition the study forms a basis fo...Continue Reading

References

Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R S YadavC J Howarth
Jan 6, 2007·Journal of Proteome Research·Bruno MeunierJean-François Hocquette
Sep 13, 2007·Molecular & Cellular Proteomics : MCP·Zhiping DengZhi-Yong Wang
May 29, 2010·Nucleic Acids Research·Sebastian BriesemeisterOliver Kohlbacher
Jul 26, 2011·Trends in Plant Science·Uwe Conrath
Dec 8, 2011·Plant Physiology·Estrella LunaJurriaan Ton
Feb 4, 2012·The Arabidopsis Book·D'Maris Amick DempseyDaniel F Klessig
Dec 4, 2012·Nucleic Acids Research·Andrea FranceschiniLars J Jensen
Aug 2, 2013·Journal of Food Science and Technology·S BalasubramanianKolhe K Onkar
Nov 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Prasad MelvinKukkundoor Ramachandra Kini
Apr 30, 2014·Nature Chemical Biology·Estrella LunaJurriaan Ton

❮ Previous
Next ❯

Citations

Apr 30, 2015·Trends in Plant Science·Andrea BalmerBrigitte Mauch-Mani
Sep 11, 2019·Bioengineered·Archana JainJingshan Shi
Apr 9, 2020·Frontiers in Plant Science·Kátia Ferreira PossaLeonor Guerra-Guimarães
May 8, 2021·Biological Reviews of the Cambridge Philosophical Society·Ashish Kumar SrivastavaPenna Suprasanna

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.