Proteomic Analysis of Epithelial to Mesenchymal Transition (EMT) Reveals Cross-talk between SNAIL and HDAC1 Proteins in Breast Cancer Cells.

Molecular & Cellular Proteomics : MCP
Camila de Souza PalmaVitor M Faça

Abstract

Epithelial to mesenchymal transition (EMT)(1) occurs naturally during embryogenesis, tissue repair, cancer progression, and metastasis. EMT induces cellular and microenvironmental changes resulting in loss of epithelial and acquisition of mesenchymal phenotypes, which promotes cellular invasive and migratory capabilities. EMT can be triggered by extracellular factors, including TGF-β, HGF, and EGF. Overexpression of transcription factors, such as SNAIL, SLUG, ZEB1/2, and TWIST1, also induces EMT and is correlated to cancer aggressiveness. Here, the breast adenocarcinoma cell line MCF7 was transduced with SNAIL to identify specific mechanisms controlled by this transcription factor during EMT. Overexpression of SNAIL led to EMT, which was thoroughly validated by molecular, morphological, and functional experiments. Subcellular proteome enrichment followed by GEL-LC-MS/MS was performed to provide extensive protein fractionation and in-depth proteomic analysis. Quantitative analysis relied on a SILAC strategy, using the invasive breast cancer cell line MDA-MB-231 as a reference for quantitation. Subsets of proteins enriched in each subcellular compartment led to a complementary list of 4289 proteins identified with high confidence...Continue Reading

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Citations

Nov 29, 2017·Frontiers in Oncology·De Domenico Stefania, Daniele Vergara
Aug 21, 2019·Molecular Omics·Virgínia Campos SilvestriniVítor Marcel Faça
Nov 5, 2017·Oncotarget·Sivan IzraelyIsaac P Witz
Nov 30, 2019·Analytical Cellular Pathology (Amsterdam)·Monica NeaguMarieta Costache
Mar 31, 2020·Molecular and Cellular Biochemistry·Raimundo Fernandes de Araujo JuniorLuis J Cruz
Nov 21, 2018·Frontiers in Oncology·Xiao YangYaling Tang
Oct 19, 2017·International Journal of Oncology·Ricardo Bonfim-SilvaAparecida Maria Fontes
Apr 13, 2021·Frontiers in Oncology·Ioanna-Maria OrfanouConstantin Tamvakopoulos
Oct 29, 2021·Bioscience Reports·Pallabi DebnathSantanu Palchaudhuri

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