PMID: 19942814Nov 28, 2009Paper

Proteomic analysis of focal cerebral ischemic injury in male rats

The Journal of Veterinary Medical Science
Phil-Ok Koh

Abstract

The present study identified the proteins that are differentially expressed during ischemic brain injury. Adult male rats were performed a middle cerebral artery occlusion (MCAO) to induce cerebral ischemia, and brains were collected at 24 hr after MCAO. Protein analysis was performed on the cerebral cortex using two-dimensional gel electrophoresis. Protein spots with a greater than 3 fold change in intensity between the sham and MCAO groups were identified by mass spectrometry. Among these proteins, 60 kDa heat shock protein, dehydropyrimidinase-related protein 2, t-complex protein 1, and Rho GDP dissociation inhibitor levels were significantly increased in MCAO group compared to those of the sham group. In contrast, thioredoxin, peroxiredoxin-2, stathmin, ubiquitin carboxy-terminal hydrolase L1, guanine nucleotide-binding protein alpha, pyridoxal-5'-phosphate phosphatase, and apoplipoprotein A-I levels were significantly decreased in MCAO group. These results suggest that cerebral ischemia induces neuronal cells death by changing expression levels of several proteins.

References

Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
Nov 22, 1993·The Journal of Comparative Neurology·M PeschanskiA Sobel
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y TakagiJ Yodoi
Apr 26, 2001·Apoptosis : an International Journal on Programmed Cell Death·M P MattsonC Culmsee
Jan 17, 2002·Brain Research·Jae Hoon BahnSoo Young Choi
Oct 3, 2003·The Journal of Biological Chemistry·Young Min JangOh-Shin Kwon
Jan 28, 2004·Cell·Keith Burridge, Krister Wennerberg
Feb 11, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kunlin JinDavid A Greenberg
Nov 3, 2004·Trends in Cell Biology·Christoph SpiessJudith Frydman
Jan 5, 2005·Genes & Development·Eve-Ellen GovekLinda Van Aelst
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Taku SugawaraPak H Chan
Oct 26, 2005·Biochemical Society Transactions·A Hall
Mar 18, 2006·Apoptosis : an International Journal on Programmed Cell Death·H ShenQ Y Liu
Dec 12, 2007·Biochemical and Biophysical Research Communications·Jinhua SunRoujia Zhou
Feb 5, 2008·Current Atherosclerosis Reports·Mohamad NavabDaniel Marelli
Jun 7, 2008·Journal of Biomedical Science·Ning Shi, Man-Ping Wu

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Citations

Aug 12, 2010·Journal of Neurotrauma·Andrew K OttensJitendra R Dave
Mar 8, 2012·Journal of Neurochemistry·Fabio Di DomenicoD Allan Butterfield
Feb 7, 2012·Journal of Neurochemistry·Antonio J López-FarréCarlos Macaya
Mar 22, 2018·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·A R GodunovaSh G Musin
Jul 15, 2017·Oxidative Medicine and Cellular Longevity·Juan Ignacio RomeroFrancisco Capani
Jan 20, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Yvonne DöringChristian Weber
Sep 13, 2012·Expert Review of Proteomics·Carlos M LabordeMaria G Barderas
Mar 26, 2014·Journal of Neurochemistry·Mireia Campos-MartorellJoan Montaner
Jul 7, 2019·Translational Stroke Research·Haiying LiGang Chen
Feb 1, 2020·Neurochemical Research·Jifei LiuZhenchang Zhang
Mar 16, 2021·The Journal of Veterinary Medical Science·Sang-A GimPhil-Ok Koh

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