Proteomic analysis of follicular fluid in carriers and non-carriers of the Trio allele for high ovulation rate in cattle

Reproduction, Fertility, and Development
Mamat H KamalludinBrian W Kirkpatrick

Abstract

This study was conducted to characterise differences in follicular fluid proteins between carriers and non-carriers of a bovine allele for high ovulation rate. A total of four non-carrier and five carrier females were used in an initial study with four and six additional non-carriers and carriers respectively used in a validation study. Emergence of the follicular wave was synchronised and the ovaries containing the dominant follicle(s) were extracted by ovariectomy for follicular fluid collection. A hexapeptide ligand library was used to overcome the masking effect of high-abundance proteins and to increase detection of low-abundance proteins in tandem mass spectrometry. After correcting for multiple comparisons, only two proteins, glia-derived nexin precursor (SERPINE2) and inhibin β B chain precursor (INHBB), were significantly differentially expressed (false-discovery rate <0.05). In a replicate study of analogous design differential expression was confirmed (P<0.05). Joint analysis of results from the two studies indicated that three additional proteins were consistently differentially expressed between genotypes. For three of these five, previous studies have indicated that expression is increased by transforming growth f...Continue Reading

References

Mar 1, 1974·Journal of Reproduction and Fertility·R G Edwards
Jul 25, 2000·Biology of Reproduction·O J GintherK Kot
Sep 27, 2002·Experimental Biology and Medicine·Corrine WeltAlan Schneyer
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Sep 29, 2004·Molecular and Cellular Endocrinology·Craig A HarrisonWylie Vale
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Mingju CaoChristopher A Price
Jun 2, 2006·Genome Biology·Ruby H P LawJames C Whisstock
Aug 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Phil G Knight, Claire Glister
Aug 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·M A Beg, O J Ginther
Oct 28, 2006·Biochimica Et Biophysica Acta·Stefania AngelucciCarmine Di Ilio
Feb 27, 2007·Journal of Chromatography. a·Egisto BoschettiPier Giorgio Righetti
Jul 8, 2008·Journal of Proteomics·Egisto Boschetti, Pier Giorgio Righetti
Jul 16, 2008·Journal of Proteomics·Xiangming Fang, Wei-Wei Zhang
Oct 15, 2009·Molecular Human Reproduction·Stephen G Hillier
Feb 19, 2010·Biology of Reproduction·Raymond J Rodgers, Helen F Irving-Rodgers
Aug 4, 2010·Journal of Proteome Research·Chengjian TuDaniel C Liebler
Aug 21, 2010·Reproduction in Domestic Animals = Zuchthygiene·S FahiminiyaN Gérard
Nov 3, 2010·Reproductive Biology and Endocrinology : RB&E·Somayyeh FahiminiyaNadine Gérard
Jan 20, 2011·Proteomics·Santosh RenuseAkhilesh Pandey
Jun 15, 2011·Molecular and Cellular Endocrinology·Phil G KnightClaire Glister
Sep 20, 2011·Proteome Science·Somayyeh FahiminiyaNadine Gérard
Jan 12, 2012·Blood·Marie-Christine BoutonMartine Jandrot-Perrus
May 28, 2013·Journal of Proteomics·Aditi S AmbekarSrabani Mukherjee
Jul 23, 2014·Endocrine Reviews·Yogeshwar MakanjiTeresa K Woodruff
Oct 31, 2014·Nature Methods·Alexey I Nesvizhskii
Jan 31, 2015·Endocrinology·Junko Nio-KobayashiWilliam Colin Duncan
Apr 4, 2015·Clinical Proteomics·Alberuni M ZamahKatherine E Williams
Jun 6, 2015·PloS One·Brian W Kirkpatrick, Chris A Morris
Aug 16, 2015·Reproductive Biology and Endocrinology : RB&E·Sheng-Hsiang LiRobert Kuo-Kuang Lee

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Citations

Dec 13, 2019·Journal of Animal Science and Biotechnology·Victor M PaesJean M Feugang
Apr 11, 2021·Journal of Proteomics·André M AlmeidaMaya Zachut

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