Proteomic analysis of hemangioblastoma cyst fluid

Cancer Biology & Therapy
S GläskerZ Zhuang

Abstract

Hemangioblastomas are benign CNS tumors that occur sporadically or in patients with von Hippel-Lindau (VHL) disease. These tumors are characteristically associated with formation of intra- or peritumoral cysts. Hemangioblastoma cyst formation is a major cause of morbidity and mortality with these tumors. While peritumoral cysts have been suggested to result from vascular leakage, the mechanism of intratumoral cyst formation is not understood. To elucidate the origin of intratumoral hemangioblastoma cyst fluid, we characterized its biochemical composition by two-dimensional (2D) proteomic profiling followed by sequencing of several proteins. The proteomic pattern of intratumoral cyst fluid was furthermore compared to the proteomic pattern of serum, hemangioblastoma tumor tissue, and hemangioblastoma peritumoral cyst fluid. We show that proteomic patterns of intra- and peritumoral cyst fluid are identical Both are highly similar to serum and not to tumor. Intratumoral hemangioblastoma cyst fluid originates from serum. Cyst formation associated with hemangioblastoma, whether peri- or intratumoral, is a consequence of vascular leakage. Anti-VEGF therapy may effectively control hemangioblastoma cyst formation.

Citations

Oct 12, 2010·Journal of Neurosurgery·Joshua J WindRussell R Lonser
Jul 7, 2007·Neurosurgical Focus·Martin A BaggenstosRussell R Lonser
Aug 27, 2014·Clinical Neurology and Neurosurgery·Chen-Chieh Liao, Yu-Hua Huang
Jun 8, 2012·FEBS Letters·Hannah L Bader, Tien Hsu
Sep 20, 2007·International Journal of Experimental Pathology·Mahmoud R Hussein
Jul 4, 2015·Journal of Neurosurgery·Ryan F HerdeAnne G Osborn
Feb 24, 2015·The Journal of Craniofacial Surgery·Quanmin NieYongming Qiu

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