Proteomic analysis of human glioblastoma cell lines differently resistant to a nitric oxide releasing agent

Molecular BioSystems
Roberta LeoneCecilia Gelfi

Abstract

Glioblastoma multiforme is the most aggressive astrocytoma characterized by the development of resistant cells to various cytotoxic stimuli. Nitric oxide (NO) is able to overcome tumor resistance in PTEN mutated rat C6 glioma cells due to its ability to inhibit cell growth by influencing the intracellular distribution of ceramide. The aim of this study is to monitor the effects of NO donor PAPANONOate on ceramide trafficking in human glioma cell lines, CCF-STTG1 (PTEN-mutated, p53-wt) and T98G (PTEN-harboring, p53-mutated), together with the assessment of their differential molecular signature by 2D-DIGE and MALDI mass spectrometry. In the CCF-STTG1 cell line, the results indicate that treatment with PAPANONOate decreased cell proliferation (<50%) and intracellular trafficking of ceramide, assessed by BODIPY-C5Cer, while these events were not observed in the T98G cell line. Proteomic results suggest that CCF-STTG1 cells are characterized by an increased expression of proteins involved in NO-associated ER stress (i.e. protein disulfide-isomerase A3, calreticulin, 78 kDa glucose-regulated protein), which could compromise ceramide delivery from ER to Golgi, leading to ceramide accumulation in ER and partial growth arrest. Converse...Continue Reading

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Citations

Feb 18, 2016·Fitoterapia·Thangavelu Soundara RajanEmanuela Mazzon
Jun 20, 2019·International Journal of Molecular Sciences·Pietro BarbaciniCecilia Gelfi
Feb 23, 2019·Biomedical Microdevices·R Araujo-GutierrezJ S Fernandez-Moure
Nov 25, 2020·Journal of Molecular Neuroscience : MN·Nabanita RoyPankaj Barah

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