Proteomic analysis of human serum from diabetic retinopathy.

International Journal of Ophthalmology
Yin-Ping LiuXiao-He Lu

Abstract

To establish and compare serum proteomic of diabetic retinopathy (DR) patients in various phases and discuss pathogenesis of DR so as to find out possible serum specific molecular markers for early diagnosis of DR. Thirty-two subjects were divided into four groups: one group of eight type 2 diabetes mellitus (T2DM) patients without apparent DR (No-DR, NDR), one group of eight T2DM patients with non-proliferative diabetic retinopathy (NPDR), one group of eight T2DM patients with proliferative diabetic retinopathy (PDR) and one group of eight healthy volunteer participants. Two dimensional fluorescence difference gel electrophoresis (2D-DIGE) was applied to establish differential protein expression profiles in four groups. Matrix-assisted laser desorption/ionization time of flight tandem mass spectrometry (MALDI-TOF-TOF MS) was applied to identify mass spectrometry of differential proteins and analyze follow-up bioinformatics. 2D-DIGE maps of serum protein were satisfactory obtained from NDR, NPDR, PDR and normal control groups. Twenty-six different proteins spots were screened (the volume ratio was >1.5 based on DeCyder software analysis). Twenty-four of them were verified and two of them were not. Fifteen proteins were verified...Continue Reading

Citations

May 5, 2012·Oman Journal of Ophthalmology·Beuy Joob, Viroj Wiwanitkit
Jun 18, 2016·Frontiers in Physiology·Priyia PusparajahKhalid Abdul Kadir
Oct 26, 2016·Current Diabetes Reports·Daniel Shu Wei TingTien Yin Wong

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