PMID: 11340594May 8, 2001Paper

Proteomic analysis of human skin fibroblasts grown on titanium: novel approach to study molecular biocompatibility

Journal of Biomedical Materials Research
K DerhamiP G Scott

Abstract

Despite the growing use of titanium as an implant material, there is a lack of consensus on what constitutes the molecular basis of its biocompatibility. In the present study we compared the response of skin fibroblasts to two different growth supporting surfaces: commercially pure titanium (cpTi) and tissue culture polystyrene (TCPS). Proteins from extracts of whole cells and adsorbed serum were separated and identified by 2-dimensional gel electrophoresis. In all, 40 proteins (46 spots) were identified by matrix-assisted laser desorption/ionization and mass spectrometry, database searching, immunoblotting, running a standard, or a combination of these techniques. Many of the proteins collected from the two surfaces were found to derive from the serum used in the culture medium. The surface properties of titanium appeared to promote the formation of a more concentrated carpet of serum proteins. Several proteins from bovine or human serum, such as albumin, alpha2-HS-glycoprotein, alpha-fetoprotein, plasminogen, thrombospondin 1, and serotransferrin, along with a few unidentified serum components, were found to adsorb onto cpTi in comparatively high concentrations. The adsorption of serum proteins did not appear to be selective ...Continue Reading

References

Jan 1, 1978·International Review of Cytology·F Grinnell
Nov 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W M BrownK M Dziegielewska
Sep 1, 1992·The American Journal of Physiology·Z Nie
Aug 1, 1991·Biomaterials·J E Ellingsen
Oct 1, 1989·Medical Hypotheses·D M Gersten, V J Hearing
May 1, 1986·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·T Nakano, P G Scott
Sep 1, 1988·Analytical Biochemistry·D F HochstrasserC R Merril
May 1, 1986·The Journal of Prosthetic Dentistry·S M ParelG Gion
Jan 1, 1986·Scandinavian Journal of Plastic and Reconstructive Surgery·C G HagertL Irstam
Mar 1, 1969·Journal of Biomedical Materials Research·R E Baier, R C Dutton
Mar 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T OhnishiY Daikuhara
Jun 11, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M C MeazziniL C Gerstenfeld

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Citations

Jul 3, 2009·Journal of the Royal Society, Interface·Laura E McNamaraRichard Burchmore
Sep 17, 2013·Journal of Cellular Physiology·Taia M B RezendeOctávio L Franco
Aug 6, 2005·Journal of Biomedical Materials Research. Part a·Jin-Ku KimDonald L Elbert
May 18, 2016·International Journal of Molecular Sciences·Zohaib KhurshidIhtesham Ur Rehman
Jul 11, 2006·The Journal of Clinical Endocrinology and Metabolism·Lucia PuricelliPaolo Tessari

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