Proteomic analysis of human Sonic Hedgehog (SHH) medulloblastoma stem-like cells

Molecular BioSystems
Maurizio RonciAndrea Urbani

Abstract

Human medulloblastoma (MB) is a malignant brain tumor that comprises four distinct molecular subgroups including the Sonic Hedgehog (SHH)-MB group. A leading cause of the SHH subgroup is an aberrant activation of the SHH pathway, a developmental signaling that regulates postnatal development of the cerebellum by promoting the mitotic expansion of granule neural precursors (GNPs) in the external granule layer (EGL). The abnormal SHH signaling pathway drives not only SHH-MB but also its cancer stem-like cells (SLCs), which represent a fraction of the tumor cell population that maintain cancer growth and have been associated with high grade tumors. Here, we report the first proteomic analysis of human SHH-MB SLCs before and after Retinoic Acid (RA)-induced differentiation. A total of 994 nLC-MS buckets were statistically analysed returning 68 modulated proteins between SLCs and their differentiated counterparts. Heat Shock Protein 70 (Hsp70) was one of the proteins that characterized the protein profile of SLCs. By means of Ingenuity Pathway Analysis (IPA), Genomatix analysis and extending the network obtained using the differentially expressed proteins we found a correlation between Hsp70 and the NF-κB complex. A key driver of th...Continue Reading

References

Mar 26, 2003·Clinical & Experimental Metastasis·Keping Xie, Suyun Huang
Apr 28, 2005·Virchows Archiv : an International Journal of Pathology·Xavier DolcetXavier Matias-Guiu
Nov 23, 2006·Nature Cell Biology·Lucia Di MarcotullioAlberto Gulino
May 31, 2007·Journal of Biosciences·Richa AryaSubhash C Lakhotia
Feb 10, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Uri TaboriCynthia Hawkins
Jun 29, 2010·The EMBO Journal·Agnese PoAlberto Gulino
Sep 9, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paul A NorthcottMichael D Taylor
Nov 26, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Yoon-Jae ChoScott L Pomeroy
Dec 21, 2010·Cancer Research·Igor ShatsJoseph R Nevins
Dec 3, 2011·Acta Neuropathologica·Michael D TaylorStefan M Pfister
Dec 23, 2011·Current Opinion in Pediatrics·Sarah E S Leary, James M Olson
Apr 5, 2012·The Journal of Biological Chemistry·Kaitlyn E Ryan, Chin Chiang
May 9, 2012·Nature Reviews. Neurology·Paul A NorthcottMichael D Taylor
Jul 17, 2012·Cancer Letters·Branavan ManoranjanSheila K Singh
Jul 27, 2012·Nature·Paul A NorthcottMichael D Taylor
Jul 27, 2012·Nature·David T W JonesPeter Lichter
Nov 24, 2012·Nature Reviews. Cancer·Paul A NorthcottStefan M Pfister
Nov 28, 2012·Experimental Neurology·Jun Wang, Robert J Wechsler-Reya
Sep 3, 2013·Journal of Neurosurgery. Pediatrics·George A AlexiouNeofytos Prodromou
Dec 24, 2013·Cancers·Kata JuhaszZsolt Balogi
Mar 22, 2014·Cancer Cell·Marcel KoolUNKNOWN ICGC PedBrain Tumor Project
May 23, 2014·Apoptosis : an International Journal on Programmed Cell Death·Lianghua DingXiaoliang Sun
Nov 14, 2014·Cancer Research·Claudia C FariaJames T Rutka
Nov 25, 2014·Cell and Tissue Research·Fredrik J SwartlingAnders I Persson

❮ Previous
Next ❯

Citations

Mar 22, 2016·Human Pathology·Natalia GarciaKatia Candido Carvalho
Feb 18, 2016·Stem Cells International·Giuseppina CatanzaroElisabetta Ferretti
Apr 15, 2015·Journal of Molecular and Cellular Cardiology·Edward G Lakatta
Mar 31, 2017·Cell Communication and Signaling : CCS·Wolfgang GruberChristian G Huber
May 29, 2018·Proteomics. Clinical Applications·Maurizio RonciMario Bonomini
Feb 9, 2020·Journal of Clinical Pathology·Hisham F Bahmad, Robert J Poppiti
Dec 21, 2017·International Journal of Molecular Sciences·Giuseppina CatanzaroElisabetta Ferretti
Aug 12, 2018·International Journal of Molecular Sciences·Agnese PoZein Mersini Besharat
Nov 23, 2019·International Journal of Molecular Sciences·Rebeca Piatniczka IglesiaMarilene Hohmuth Lopes
Mar 13, 2020·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Martina ChiacchiariniElisabetta Ferretti
Jan 8, 2021·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Riccardo Serra, Antonella Mangraviti
Aug 2, 2021·Cellular and Molecular Life Sciences : CMLS·Mariana MedeirosMaría Sol Brassesco

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cancer -Omics

A variety of different high-throughput technologies can be used to identify the complete catalog of changes that characterize the molecular profile of cohorts of tumor samples. Discover the latest insights gained from cancer 'omics' in this feed.