Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis

Clinical Proteomics
Ji-Hyun LeeJong-Seo Kim

Abstract

Ankylosing spondylitis (AS) is a chronic inflammatory rheumatic disease affecting the axial skeleton and peripheral joints. The etiology of this disease remains poorly understood, but interactions between genetic and environmental factors have been implicated. The present study identified differentially expressed proteins in the synovial fluid (SF) of AS patients to elucidate the underlying cause of AS. A cohort of 40 SF samples from 10 AS and 10 each of rheumatoid arthritis (RA), gout, and osteoarthritis (OA) patients were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify differentially expressed proteins specific to AS. The label-free LC-MS/MS results were verified by western blotting. We identified 8 proteins that were > 1.5-fold upregulated in the SF of AS patients compared to that of the disease control groups, including HP, MMP1, MMP3, serum amyloid P-component (APCS), complement factor H-related protein 5 (CFHR5), mannose-binding lectin 2 (MBL2), complement component C9 (C9), and complement C4-A (C4A). CFHR5 and C9 were previously found in serum from AS patients, while APCS was previously found in SF as well as in serum. However, the present study has identified C4A, and MBL2 as potential ...Continue Reading

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Citations

Oct 14, 2021·Database : the Journal of Biological Databases and Curation·Dan ShaoJuan Cui
Nov 1, 2021·Journal of Proteomics·Yuxiu SunAnlong Xu

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Methods Mentioned

BETA
imaging techniques
immunodepletion
acetylation
electrophoresis
proteomic profiling

Software Mentioned

Maxquant
Cytoscape
DAVID
Perseus
MaxLFQ

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