Proteomic analysis of hypoxia and non-hypoxia secretome mesenchymal stem-like cells from human breastmilk.

Saudi Journal of Biological Sciences
Sri Lilidjanti Widjaja Soetrisno


Breastmilk contains proteins and cells which have stem cell properties. The human breastmilk stem cell mimick mesenchymal stem cells and expresses pluripotency genes. The protein level of breastmilk is high in colostrum and gradually subsides in the first year of lactation. The mesenchymal stem cells from breastmilk can be an alternative source of stem cells that can potentially affect cardiovascular therapy. This study aimed to identify the proteomic analysis of secretome mesenchymal stem-like cells under hypoxia compared to non-hypoxia from human breastmilk stem cells. The human breastmilk was collected from six healthy breastfeeding women and transported to the laboratory under aseptic conditions. The breastmilk cells were isolated then cultured. After 72 h, the human breastmilk stem cells reached confluence then cleaned up and isolated in serum-free media (spheroid) to allow serial passaging every 48 h. The acquisition stem cell was made with flow cytometry. The cells were divided into hBSC secretomes under hypoxia (A) and non-hypoxia (B) and analyzed for LC-MS to identify the peptide structure. The human breastmilk cells contained several mesenchymal stem-like cells in density 2.4 × 106 cell/mL for hypoxia and 2 × 106 cell...Continue Reading


Jun 9, 2004·International Journal of Cancer. Journal International Du Cancer·Mariko ShimamuraMasaaki Iigo
May 10, 2005·Journal of Cellular Physiology·Victor VacantiTechung Lee
Apr 19, 2007·Cell and Tissue Research·Mark D CreganPeter E Hartmann
Aug 18, 2010·Human Cell·Satish PatkiRamesh Bhonde
May 25, 2011·World Journal of Stem Cells·Farbod RastegarTong-Chuan He
Dec 15, 2011·Molecular and Cellular Biochemistry·Dan GuoJianping Gong
Feb 16, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yalin LiaoBo Lönnerdal
Aug 7, 2012·Stem Cells·Foteini HassiotouPeter E Hartmann
Sep 5, 2012·Journal of Translational Medicine·Lenka KubiczkovaSabina Sevcikova
Sep 25, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Elena T ZakharovaVadim B Vasilyev
Nov 28, 2012·Pediatric Clinics of North America·Olivia Ballard, Ardythe L Morrow
Dec 17, 2014·Neurology Research International·Seyed Mojtaba HosseiniBahareh Owrangi
Mar 24, 2015·Bioscience Reports·Imran UllahGyu Jin Rho
Aug 11, 2015·Scientific Reports·Alecia-Jane TwiggerFoteini Kakulas
Oct 18, 2016·Advances in Neonatal Care : Official Journal of the National Association of Neonatal Nurses·Carrie-Ellen BriereChristine Finck
Oct 19, 2016·IUBMB Life·Somia H Abd AllahHesham M Kelani
Jan 20, 2017·Annals of Nutrition & Metabolism·Ferdinand HaschkeSagar K Thakkar
Aug 26, 2017·International Journal of Molecular Sciences·Francisco J VizosoRoman Perez-Fernandez
Apr 12, 2019·Human Cell·Natalia NinkinaVladimir L Buchman
Apr 15, 2020·Nutrients·Katríona E LyonsCatherine Stanton

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Nursing Journal of India
B Belavady
Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine
Foteini KakulasPeter E Hartmann
British Dental Journal
O Barratt, E Brewer
Cell and Tissue Banking
Seema TripathySaroj Kumar Das
© 2021 Meta ULC. All rights reserved