Proteomic analysis of hypoxia and non-hypoxia secretome mesenchymal stem-like cells from human breastmilk.

Saudi Journal of Biological Sciences
Sri Lilidjanti Widjaja Soetrisno

Abstract

Breastmilk contains proteins and cells which have stem cell properties. The human breastmilk stem cell mimick mesenchymal stem cells and expresses pluripotency genes. The protein level of breastmilk is high in colostrum and gradually subsides in the first year of lactation. The mesenchymal stem cells from breastmilk can be an alternative source of stem cells that can potentially affect cardiovascular therapy. This study aimed to identify the proteomic analysis of secretome mesenchymal stem-like cells under hypoxia compared to non-hypoxia from human breastmilk stem cells. The human breastmilk was collected from six healthy breastfeeding women and transported to the laboratory under aseptic conditions. The breastmilk cells were isolated then cultured. After 72 h, the human breastmilk stem cells reached confluence then cleaned up and isolated in serum-free media (spheroid) to allow serial passaging every 48 h. The acquisition stem cell was made with flow cytometry. The cells were divided into hBSC secretomes under hypoxia (A) and non-hypoxia (B) and analyzed for LC-MS to identify the peptide structure. The human breastmilk cells contained several mesenchymal stem-like cells in density 2.4 × 106 cell/mL for hypoxia and 2 × 106 cell...Continue Reading

References

Jun 9, 2004·International Journal of Cancer. Journal International Du Cancer·Mariko ShimamuraMasaaki Iigo
May 10, 2005·Journal of Cellular Physiology·Victor VacantiTechung Lee
Apr 19, 2007·Cell and Tissue Research·Mark D CreganPeter E Hartmann
Aug 18, 2010·Human Cell·Satish PatkiRamesh Bhonde
May 25, 2011·World Journal of Stem Cells·Farbod RastegarTong-Chuan He
Dec 15, 2011·Molecular and Cellular Biochemistry·Dan GuoJianping Gong
Feb 16, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yalin LiaoBo Lönnerdal
Aug 7, 2012·Stem Cells·Foteini HassiotouPeter E Hartmann
Sep 5, 2012·Journal of Translational Medicine·Lenka KubiczkovaSabina Sevcikova
Sep 25, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Elena T ZakharovaVadim B Vasilyev
Nov 28, 2012·Pediatric Clinics of North America·Olivia Ballard, Ardythe L Morrow
Dec 17, 2014·Neurology Research International·Seyed Mojtaba HosseiniBahareh Owrangi
Mar 24, 2015·Bioscience Reports·Imran UllahGyu Jin Rho
Aug 11, 2015·Scientific Reports·Alecia-Jane TwiggerFoteini Kakulas
Oct 18, 2016·Advances in Neonatal Care : Official Journal of the National Association of Neonatal Nurses·Carrie-Ellen BriereChristine Finck
Oct 19, 2016·IUBMB Life·Somia H Abd AllahHesham M Kelani
Jan 20, 2017·Annals of Nutrition & Metabolism·Ferdinand HaschkeSagar K Thakkar
Aug 26, 2017·International Journal of Molecular Sciences·Francisco J VizosoRoman Perez-Fernandez
Apr 12, 2019·Human Cell·Natalia NinkinaVladimir L Buchman
Apr 15, 2020·Nutrients·Katríona E LyonsCatherine Stanton

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