Proteomic analysis of iron acquisition, metabolic and regulatory responses of Yersinia pestis to iron starvation.

BMC Microbiology
Rembert PieperScott N Peterson

Abstract

The Gram-negative bacterium Yersinia pestis is the causative agent of the bubonic plague. Efficient iron acquisition systems are critical to the ability of Y. pestis to infect, spread and grow in mammalian hosts, because iron is sequestered and is considered part of the innate host immune defence against invading pathogens. We used a proteomic approach to determine expression changes of iron uptake systems and intracellular consequences of iron deficiency in the Y. pestis strain KIM6+ at two physiologically relevant temperatures (26 degrees C and 37 degrees C). Differential protein display was performed for three Y. pestis subcellular fractions. Five characterized Y. pestis iron/siderophore acquisition systems (Ybt, Yfe, Yfu, Yiu and Hmu) and a putative iron/chelate outer membrane receptor (Y0850) were increased in abundance in iron-starved cells. The iron-sulfur (Fe-S) cluster assembly system Suf, adapted to oxidative stress and iron starvation in E. coli, was also more abundant, suggesting functional activity of Suf in Y. pestis under iron-limiting conditions. Metabolic and reactive oxygen-deactivating enzymes dependent on Fe-S clusters or other iron cofactors were decreased in abundance in iron-depleted cells. This data was ...Continue Reading

References

Oct 1, 1988·Analytical Biochemistry·L H Johansson, L A Borg
Mar 20, 1967·Archives of Biochemistry and Biophysics·I A Rose, E L O'Connell
Sep 12, 1997·The Journal of Biological Chemistry·E EichhornT Leisinger
May 7, 1999·Molecular Microbiology·J D FetherstonR D Perry
Jun 11, 1999·Molecular Microbiology·C M SzymanskiP Guerry
Jan 6, 2000·Archives of Biochemistry and Biophysics·D Touati
Mar 4, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·A V Peskin, C C Winterbourn
Nov 22, 2000·The Biochemical Journal·G Cairo, A Pietrangelo
Dec 6, 2000·The Biochemical Journal·Y Y Chang, J E Cronan
Apr 3, 2001·Current Opinion in Microbiology·K Hantke
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eric Massé, Susan Gottesman
Jul 27, 2002·Journal of Bacteriology·Wen DengRobert D Perry
Dec 18, 2002·Journal of Bacteriology·Shery VargheseJames A Imlay
May 7, 2003·Current Opinion in Microbiology·Patricia J Kiley, Helmut Beinert
May 22, 2003·Advances in Experimental Medicine and Biology·Robert D PerryJacqueline D Fetherston
Jun 26, 2003·Nucleic Acids Research·Jacques van Helden
Oct 7, 2003·Annual Review of Microbiology·James A Imlay
Mar 5, 2004·Journal of Bacteriology·Robert D PerryJacqueline D Fetherston
Apr 23, 2004·Molecular Microbiology·F Wayne OuttenGisela Storz
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Paula J WildermanMichael L Vasil
Jun 25, 2004·Nucleic Acids Research·Pantelis G BagosStavros J Hamodrakas
Jul 24, 2004·The Journal of Infectious Diseases·Clayton O JarrettB Joseph Hinnebusch
Nov 20, 2004·Nature Reviews. Microbiology·Ulrich E Schaible, Stefan H E Kaufmann
May 3, 2005·Nature Reviews. Microbiology·Agnès VendevilleKim R Hardie
Sep 27, 2005·Microbes and Infection·Dongsheng ZhouRuifu Yang
Oct 4, 2005·Journal of Bacteriology·Robert J Kadner
Feb 14, 2006·Proteomics·Christine L GatlinScott N Peterson
Apr 25, 2006·FEMS Microbiology Letters·Dongsheng ZhouRuifu Yang
Jul 26, 2006·The Journal of Biological Chemistry·Victor W T ChengJoel H Weiner
Sep 7, 2006·Infection and Immunity·Olga KirillinaRobert D Perry
Sep 22, 2006·Molecular Microbiology·Cornelia GrosseGregor Grass
Nov 4, 2006·Journal of Proteome Research·Kim K HixsonMary S Lipton
Jan 9, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Robert D PerryJacqueline D Fetherston
Mar 14, 2007·The Journal of Biological Chemistry·Gunhild LayerF Wayne Outten
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·S GottesmanD J FitzGerald

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Citations

Oct 6, 2011·Journal of Proteome Research·Juliana CrestaniMarilene Henning Vainstein
Jun 1, 2010·Journal of Bacteriology·Christophe S BernardEric Cascales
Feb 9, 2016·Biochimica Et Biophysica Acta·Navatha AlugubellyMariola J Edelmann
Dec 18, 2013·Molecular and Cellular Probes·David WunschelKaren Wahl
Oct 8, 2016·Veterinary Research·Gokhlesh KumarMansour El-Matbouli
Apr 22, 2015·Antimicrobial Agents and Chemotherapy·Danielle B SteedFloyd E Romesberg
Oct 21, 2020·Current Protocols in Microbiology·Robert K Davidson, Kimberly M Davis

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Methods Mentioned

BETA
membrane filtration
enzyme
electrophoresis
proteomic profiling

Software Mentioned

Mascot
TMBB
TMHMM
- matrix
PSORTb
PRED
Proteomweaver
patser

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