Proteomic Analysis of Kiwifruit in Response to the Postharvest Pathogen, Botrytis cinerea

Frontiers in Plant Science
Jia LiuYongsheng Liu

Abstract

Gray mold, caused by the fungus Botrytis cinerea, is the most significant postharvest disease of kiwifruit. In the present study, iTRAQ with LC-ESI-MS/MS was used to identify the kiwifruit proteins associated with the response to B. cinerea. A total of 2,487 proteins in kiwifruit were identified. Among them, 292 represented differentially accumulated proteins (DAPs), with 196 DAPs having increased, and 96 DAPs having decreased in accumulation in B. cinerea-inoculated vs. water-inoculated, control kiwifruits. DAPs were associated with penetration site reorganization, cell wall degradation, MAPK cascades, ROS signaling, and PR proteins. In order to examine the corresponding transcriptional levels of the DAPs, RT-qPCR was conducted on a subset of 9 DAPs. In addition, virus-induced gene silencing was used to examine the role of myosin 10 in kiwifruit, a gene modulating host penetration resistance to fungal infection, in response to B. cinerea infection. The present study provides new insight on the understanding of the interaction between kiwifruit and B. cinerea.

References

Sep 7, 2000·Molecular Plant-microbe Interactions : MPMI·A L PowellJ M Labavitch
Oct 24, 2008·Phytopathology·Eleni K KulakiotuEvangelos M Sfakiotakis
Jan 17, 2009·Biochemical and Biophysical Research Communications·Dong Sook LeeOhkmae K Park
Sep 1, 2007·Molecular Plant Pathology·Brian WilliamsonJan A L van Kan
Jan 17, 2012·Current Biology : CB·Karolina M Pajerowska-MukhtarXinnian Dong
Mar 1, 2012·Journal of Proteome Research·Punit ShahGerardo Gutierrez-Sanchez
May 15, 2013·Annual Review of Phytopathology·Xiangzong Meng, Shuqun Zhang
Jun 28, 2013·The Plant Journal : for Cell and Molecular Biology·Qian LiPing Leng
Jul 23, 2013·Current Opinion in Plant Biology·Aardra Kachroo, Guillaume P Robin
Mar 19, 2014·Nature Communications·Rania GhossoubPascale Zimmermann
Jul 9, 2014·Annual Review of Phytopathology·Christian P KubicekN Louise Glass
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Long YangYangdou Wei
May 24, 2015·Environmental Science and Pollution Research International·Huizhen ChenJia Liu
Oct 27, 2015·Frontiers in Plant Science·Raquel González-FernándezJesús V Jorrín-Novo
Mar 8, 2016·Plant Physiology and Biochemistry : PPB·Daymi CamejoAlexander Moreno
Mar 24, 2016·Journal of Proteomics·Eva LiñeiroFrancisco Javier Fernández-Acero
Sep 30, 2016·Scientific Reports·Lihong LiuQiaomei Wang
Sep 30, 2016·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Nov 24, 2016·Current Protein & Peptide Science·Jahangir ImamMukund Variar
Jan 11, 2017·Journal of Experimental Botany·Li ZhangSheng Yang He
Feb 1, 2017·Critical Reviews in Food Science and Nutrition·Jia LiuQi Wang

❮ Previous
Next ❯

Datasets Mentioned

BETA
PXD008589

Methods Mentioned

BETA
PCR
GTPase
transgenic

Software Mentioned

Mascot
Proteome Discoverer

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved