Proteomic analysis of laser-microdissected paraffin-embedded tissues: (2) MRM assay for stage-related proteins upon non-metastatic lung adenocarcinoma

Journal of Proteomics
Toshihide NishimuraHarubumi Kato

Abstract

A preceding paper suggested 81 candidates of stage-specifically expressed proteins for either stage IA or IIIA by global shotgun proteomics and spectral counting. Six proteins, a subset of these proteins, were chosen for a further verification study since they are potentially soluble and/or secretory, which nature is convenient for detecting them in blood in clinical practice. The multiple-reaction monitoring (MRM) quantitative analysis suggested that napsin-A and anterior gradient protein 2 homolog (hAG-2) out of the 6 candidates would be useful for determining stage IA or IIIA and are related to metastasis. In the study we noted that stage IIIA patients with better outcome showed napsin-A profiles similar to that of stage IA patients. We therefore examined 14 additional patients for analysis, which contained the IA-stage patients of poorer outcome and the IIIA-stage patients of better outcome. The MRM analysis of napsin-A for all patients suggests that napsin-A contents correlate with better outcome in stage IA. This and discovery studies demonstrate that direct isolation of tumor cells alone by laser microdissection (LMD) greatly reduces complexity on comprehensive analyses, and that MRM mass spectrometry using the endogenou...Continue Reading

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Citations

Dec 6, 2011·Bioanalysis·Ákos Végvári, Balázs Döme
Aug 12, 2014·Omics : a Journal of Integrative Biology·Rémi LonguespéeEdwin De Pauw
Oct 13, 2015·Expert Review of Proteomics·Vendula Pernikářová, Pavel Bouchal
May 4, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ming-Yi XuLun-Gen Lu
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Apr 27, 2010·Chemical Reviews·Akos Végvári, György Marko-Varga

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