Proteomic analysis of left ventricular tissues following intermittent myocardial ischemia during coronary collateralization in rabbits

International Journal of Cardiology
Jinyang GuTingjian Jin

Abstract

Repeated transient myocardial ischemia may offer favorable effects to coronary perfusion via collateral circulation, although the underlying molecular mechanisms still remain unclear. This study was designed to evaluate the proteomic changes during this process. Rabbits were randomly divided into sham-operated and ischemic groups (5 each) and were subjected to intermittent myocardial ischemia by inflation or deflation of pneumatic occluders for 4 weeks to establish a controlled myocardial ischemic model. Isolated hearts were subjected to histological observation, microspheric detection, capillary counting and proteomic analysis. Elevation of ST segment or back to normal in Lead-II electrocardiogram could be induced by occluders without overt histological and cardiac troponin I alterations. Regional coronary collateral blood flow exhibited a remarkable increase following intermittent inflation of occluders in the ischemic group (P<0.01). Simultaneously, capillary numbers per unit area were significantly different between groups (P<0.01). Twenty-three differentially expressed protein spots were separated by two-dimensional gel electrophoresis and 13 out of them were identified by MALDI-TOF-MS. The present study indicates that the...Continue Reading

References

Dec 1, 1992·Circulation Research·F C WhiteC M Bloor
May 1, 1990·International Journal of Cardiology·M FujitaD Franklin
Nov 18, 1971·The New England Journal of Medicine·J Folkman
Oct 1, 1994·Medicine and Science in Sports and Exercise·M D McKirnan, C M Bloor
Apr 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Krista K GravenHarrison W Farber
Mar 26, 2003·Brain Research·Xiao-Hao YaoXue-Jun Li
Jan 6, 2004·Free Radical Research·Ann van CampenhoutBegoña Manuel-y-Keenoy
Apr 22, 2004·QJM : Monthly Journal of the Association of Physicians·M H TayebjeeR J MacFadyen
Apr 29, 2004·Journal of Proteome Research·Hans-Reinhard ZerkowskiIvan Lefkovits
May 13, 2006·Journal of Bioenergetics and Biomembranes·Daniel J Kenan, Miriam L Wahl
Jul 4, 2006·Trends in Biochemical Sciences·Christian W GruberDavid J Craik
Dec 30, 2006·Circulation·Wayne RosamondUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.